Gonzalez Teresita de Jesus Bello, Marcato Francesca, de Freitas Costa Eduardo, van den Brand Henry, Hoorweg Fleur A, Wolthuis-Fillerup Maaike, Engel Bas, Schnabel Sabine K, van Reenen Cornelis G, Brouwer Michael S M
Department of Bacteriology, Host-Pathogen Interaction, and Diagnostics Development. Wageningen Bioveterinary Research, 8221 RA Lelystad, the Netherlands.
Adaptation Physiology Group, Wageningen University & Research, 6700 AH Wageningen, the Netherlands; Wageningen Livestock Research, Wageningen University & Research, 6700 AH Wageningen, the Netherlands.
Vet Microbiol. 2022 Oct;273:109520. doi: 10.1016/j.vetmic.2022.109520. Epub 2022 Jul 28.
A longitudinal study was performed to investigate the prevalence of Extended-Spectrum Cephalosporin-Resistant (ESC-R) Escherichia coli colonization in Dutch veal farms. Rectal swabs from 683 calves born in 13 Dutch dairy farms were collected one day prior to transportation to the veal farm at 14 or 28 days of age, and at 5 different time points 8 Dutch veal farms. In addition, characteristics of the calf, cows, and farm management were collected. Rectal swabs were selectively cultured for ESC-R E. coli. In total, 1202 ESC-R E. coli isolates were recovered. Overall, the prevalence of ESC-R E. coli increased from 24.4 % at one day prior to transportation to 57.3 % in week two after arrival of calves at the veal farm. No associations were found between the presence of ESC-R E. coli at the dairy or veal farm and age of transportation, sex and breed. The presence of ESC-R E. coli in week 6, 10, and 18 at the veal farm was positively associated with the presence of ESC-R E. coli in week 10, 18, and 24, respectively (p < 0.05). Individual antibiotic treatments applied before week 2 and 6 upon arrival to the veal farms tended to increase the ESC-R E. coli colonization frequency. Our results indicate that ESC-R E. coli colonization frequency substantially increases after arrival of calves on the veal farm. In addition to individual antibiotic treatments, it is considered likely that frequently applied batch antibiotic treatments are also implicated in the ESC-R E. coli colonization frequency.
进行了一项纵向研究，以调查荷兰小牛肉农场中耐超广谱头孢菌素（ESC-R）大肠杆菌定植的流行情况。在13个荷兰奶牛场出生的683头犊牛，于14或28日龄运输至小牛肉农场前一天、以及在8个荷兰小牛肉农场的5个不同时间点采集直肠拭子。此外，还收集了犊牛、母牛和农场管理的特征信息。对直肠拭子进行选择性培养以检测ESC-R大肠杆菌。总共分离出1202株ESC-R大肠杆菌。总体而言，ESC-R大肠杆菌的流行率从运输前一天的24.4%增加到犊牛抵达小牛肉农场后第二周的57.3%。在奶牛场或小牛肉农场中ESC-R大肠杆菌的存在与运输年龄、性别和品种之间未发现关联。在小牛肉农场第6、10和18周时ESC-R大肠杆菌的存在分别与第10、18和24周时ESC-R大肠杆菌的存在呈正相关（p<0.05）。在抵达小牛肉农场后第2周和第6周之前进行的个体抗生素治疗往往会增加ESC-R大肠杆菌的定植频率。我们的结果表明，犊牛抵达小牛肉农场后，ESC-R大肠杆菌的定植频率大幅增加。除个体抗生素治疗外，频繁应用的批量抗生素治疗也可能与ESC-R大肠杆菌的定植频率有关。
