利用微同步辐射 X 射线荧光（µ-SRXRF）对 MRI 对比剂进行痕量金属成像，以用于前列腺癌成像。
Using micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) for trace metal imaging in the development of MRI contrast agents for prostate cancer imaging.
机构信息
Department of Physics & Astronomy, McMaster University, 1280 Main St. W., Hamilton, ON L8S 4K1, Canada; Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tuebingen, 72076, Germany.
Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, United States; Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tuebingen, 72076, Germany.
出版信息
J Trace Elem Med Biol. 2022 Dec;74:127054. doi: 10.1016/j.jtemb.2022.127054. Epub 2022 Aug 3.
BACKGROUND
Contrast agents (CA) are administered in magnetic resonance imaging (MRI) clinical exams to measure tissue perfusion, enhance image contrast between adjacent tissues, or provide additional biochemical information in molecular MRI. The efficacy of a CA is determined by the tissue distribution of the agent and its concentration in the extracellular space of all tissues.
METHODS
In this work, micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) was used to examine and characterize a gadolinium-based zinc-sensitive agent (GdL) currently under development for detection of prostate cancer (PCa) by MRI. Prostate tissue samples were collected from control mice and mice with known PCa after an MRI exam that included injection of GdL. The samples were raster scanned to investigate trends in Zn, Gd, Cu, Fe, S, P, and Ca.
RESULTS
Significant Zn and Gd co-localization was observed in both healthy and malignant tissues. In addition, a marked decrease in Zn was found in the lateral lobe of the prostate obtained from mice with PCa.
CONCLUSION
We demonstrate here that µ-SRXRF is a useful tool for monitoring the distribution of several elements including Zn and Gd in animal models of cancer. The optimized procedures for tissue preparation, processing, data collection, and analysis are described.
背景
在磁共振成像 (MRI) 临床检查中，使用对比剂 (CA) 来测量组织灌注，增强相邻组织之间的图像对比度，或在分子 MRI 中提供额外的生化信息。CA 的功效取决于试剂在组织中的分布及其在所有组织细胞外空间中的浓度。
方法
在这项工作中，微同步辐射 X 射线荧光 (µ-SRXRF) 被用于检查和表征一种基于镓的锌敏试剂 (GdL)，该试剂目前正在开发中，用于通过 MRI 检测前列腺癌 (PCa)。在包括注射 GdL 的 MRI 检查后，从对照小鼠和已知患有 PCa 的小鼠中采集前列腺组织样本。对样本进行光栅扫描，以研究 Zn、Gd、Cu、Fe、S、P 和 Ca 的趋势。
结果
在健康和恶性组织中均观察到明显的 Zn 和 Gd 共定位。此外，在来自患有 PCa 的小鼠的前列腺侧叶中发现 Zn 明显减少。
结论
我们在这里证明，µ-SRXRF 是一种用于监测包括 Zn 和 Gd 在内的多种元素在癌症动物模型中分布的有用工具。描述了用于组织准备、处理、数据收集和分析的优化程序。
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