Jennings M G, McGowan M P, Baker H W
Clin Reprod Fertil. 1986 Dec;4(6):359-66.
An aetiological role for non-specific genital tract infection (GTI) in male infertility has long been postulated and has led to routine bacteriologic culture of semen in some laboratories. To assess the value of examination of various semen characteristics and conventional culture of semen as diagnostic criteria for GTI, frequencies of bacterial isolation and relationships with leukocytospermia and other abnormalities of semen were determined in 806 samples from 459 men seen over a 2 year period. Combinations of features suggestive of GTI were present in 19.6%. High leucocyte counts were found in 13.5% of specimens and evidence of possible accessory sex organ dysfunction in 57.7%. Significant numbers of bacteria were isolated from 19.5% of samples. Of the few pathogens cultured (46 specimens), 53% were Staphylococcus aureus, a possible contaminant. Furthermore, only one specimen had more than 10(6) pathogenic bacteria per ml, also Staph. aureus. There was a lack of correlation between the presence of bacteriospermia, leukocytospermia and semen changes possibly attributable to accessory sex organ dysfunction, shedding doubt on their relevance to identification of GTI. This was the case even in repeatedly culture-positive specimens. Conventional bacteriological examination of semen specimens is not merited as a routine procedure.
长期以来，人们一直假定非特异性生殖道感染（GTI）在男性不育中具有病因学作用，这导致一些实验室对精液进行常规细菌培养。为了评估各种精液特征检查和精液常规培养作为GTI诊断标准的价值，我们对459名男性在两年期间的806份精液样本进行了细菌分离频率测定，并确定了其与白细胞精子症及其他精液异常的关系。提示GTI的特征组合出现在19.6%的样本中。13.5%的标本白细胞计数高，57.7%有附属性腺功能可能异常的证据。19.5%的样本分离出大量细菌。在培养出的少数病原体（46份标本）中，53%是金黄色葡萄球菌，这可能是一种污染物。此外，每毫升精液中只有一份标本的病原菌超过10⁶，也是金黄色葡萄球菌。细菌精子症、白细胞精子症与可能归因于附属性腺功能障碍的精液变化之间缺乏相关性，这让人怀疑它们与GTI诊断的相关性。即使在多次培养呈阳性的标本中也是如此。精液标本的常规细菌学检查作为常规程序并不值得。
