Villanueva-Diaz C A, Flores-Reyes G A, Beltrán-Zúñiga M, Echavarría-Sánchez M, Ortiz-Ibarra F J, Arredondo-García J L
Departamento de Andrologá, México, D.F., México.
Int J Fertil Womens Med. 1999 Jun-Aug;44(4):198-203.
To test a method for increasing the sensitivity of semen culture. Design-Prospective and transverse. Setting-Andrology clinic at a tertiary care health institution in Mexico City.
65 infertile patients with abnormal semen, bacteriospermia detected on Gram stain and at least two previous negative semen cultures (<3 months) were included to test routine semen culture and a method including centrifugation of semen at 10,00 rpm for 20 minutes. Localization cultures were also carried out in all patients.
Bacterial isolation in semen samples.
Routine semen culture was positive in 22% of patients, while centrifuged aliquots of the same semen sample were positive in 52% of patients (chi2 = 6.60, P < .01). Enterococcus was isolated in 43% of patients, E. coli in 24%, coagulase-negative Staphylococcus in 19%, and U. urealyticum in 14%. Ninety percent of isolates corresponded to specimens from the urethra and the prostato-vesicular region.
Sensitivity of semen culture increased with centrifugation of semen samples. Localization pattern and type of isolates suggest that these patients had chronic prostatitis and that episodic elimination of bacteria might also explain false negative semen cultures in patients with chronic asymptomatic infection of the accessory sex glands.
测试一种提高精液培养敏感性的方法。设计——前瞻性和横向研究。地点——墨西哥城一家三级医疗保健机构的男科诊所。
纳入65例精液异常的不育患者，革兰氏染色检测到菌精症，且之前至少有两次精液培养阴性（<3个月），以测试常规精液培养以及一种包括以10,00转/分钟离心精液20分钟的方法。所有患者均进行定位培养。
精液样本中的细菌分离情况。
常规精液培养在22%的患者中呈阳性，而同一精液样本的离心等分试样在52%的患者中呈阳性（χ² = 6.60，P < .01）。43%的患者分离出肠球菌，24%分离出大肠杆菌，19%分离出凝固酶阴性葡萄球菌，14%分离出解脲脲原体。90%的分离株对应于来自尿道和前列腺 - 精囊区域的标本。
精液样本离心可提高精液培养的敏感性。分离株的定位模式和类型表明这些患者患有慢性前列腺炎，并且细菌的间歇性清除也可能解释了慢性无症状性附属性腺感染患者精液培养假阴性的原因。
