Grossgebauer K
Z Hautkr. 1983 Apr 1;58(7):498-508.
Microbiological analysis of semen in andrology is mainly performed in cases of infertility and artificial insemination. In order to localize the suggested infection and to detect urethral (asymptomatic) colonization, we recommend to analyse additional specimens of the patient, i.e. urethral swabs and first portion of urine. As a rule, quantitative microbiological analysis is necessary in cases of bacteria belonging to the group of potentially pathogenic flora. In isolating pathogenic ("specific") bacteria, the detection of a few microorganisms indicates an infectious process. We recommend the careful microscopical examination of the ejaculate to detect phagocytes, abnormal spermatozoa, trichomonads, yeasts and other cells. As a rapid microscopical examination we employ the DAPI-fluorochrome-technique. Microbiological cultures should take into consideration the isolation and identification of ureaplasmas and chlamydia trachomatis. In order to achieve a comparison of microbiological results with those of other laboratories, it is essential to define the terms "significant bacteriospermia" and "significant leukocytospermia". In other words, it is necessary to introduce a borderline between contamination and infection concerning the "nonspecific" potentially pathogenic flora.
男科精液的微生物学分析主要在不育症和人工授精病例中进行。为了定位疑似感染并检测尿道（无症状）定植情况，我们建议对患者的其他标本进行分析，即尿道拭子和初段尿液。通常，对于属于潜在致病菌群的细菌，进行定量微生物学分析是必要的。在分离致病性（“特异性”）细菌时，检测到少量微生物表明存在感染过程。我们建议对精液进行仔细的显微镜检查，以检测吞噬细胞、异常精子、滴虫、酵母和其他细胞。作为一种快速显微镜检查方法，我们采用DAPI荧光染色技术。微生物培养应考虑解脲脲原体和沙眼衣原体的分离与鉴定。为了将微生物学结果与其他实验室的结果进行比较，明确“显著菌精症”和“显著白细胞精症”的定义至关重要。换句话说，有必要在关于“非特异性”潜在致病菌群的污染和感染之间划定界限。
