Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.
J Periodontal Res. 2022 Oct;57(5):1043-1055. doi: 10.1111/jre.13045. Epub 2022 Aug 9.
To compare miRNA expression levels and predict relevant target genes and signaling pathways in peri-implantitis and periodontitis.
There are many differences between periodontitis and peri-implantitis. An understanding of the similarities and differences in the transcriptional patterns of these diseases, as well as the molecular mechanisms, is beneficial for the development of management strategies.
Rat models of periodontitis (PD, n = 6) and peri-implantitis (PI, n = 5) were established by ligation. Implantation without ligation (PIC, n = 5) and normal rats (PDC, n = 6) were used as controls. Micro-CT was used to confirm the successful establishment of the model. Gingiva was harvested for miRNA transcriptome sequencing, and the results were confirmed by qRT-PCR. miRNA target genes were predicted with miRTarBase. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed.
Sixty-nine miRNAs were differentially expressed in PI vs. PD, 105 were differentially expressed in PI vs. PIC, and 70 were differentially expressed in PD vs. PDC (log FC ≥1 and padj <0.05). The upregulated genes in all three comparisons were mostly involved in the biological process response to stimulus, whereas most of the downregulated genes were involved in nervous system development (p < .01). The upregulated genes in PI vs. PD and PI vs. PIC were involved in Toll-like receptor signaling and RIG-I-like signaling. The upregulated genes in PI vs. PD were involved in T- and B-cell receptor signaling, apoptosis, and osteoclast differentiation. Focal adhesion was downregulated in all three comparisons, and adherens junction was downregulated in PI vs. PD and PD vs. PDC (p < .1).
This study showed differences in the miRNA expression profiles between peri-implantitis and periodontitis and annotated the possible target genes and molecular mechanisms; this study could lay a foundation for the development of management strategies.
比较种植体周围炎和牙周炎中 miRNA 表达水平，预测相关靶基因和信号通路。
牙周炎和种植体周围炎有许多不同之处。了解这些疾病转录模式的异同以及分子机制，有利于制定管理策略。
结扎法建立牙周炎（PD，n=6）和种植体周围炎（PI，n=5）大鼠模型。未结扎种植体（PIC，n=5）和正常大鼠（PDC，n=6）作为对照。微 CT 用于确认模型建立成功。采集牙龈进行 miRNA 转录组测序，并用 qRT-PCR 进行验证。利用 miRTarBase 预测 miRNA 靶基因。进行基因本体（GO）和京都基因与基因组百科全书（KEGG）分析。
PI 与 PD 比较，69 个 miRNA 差异表达，PI 与 PIC 比较，105 个 miRNA 差异表达，PD 与 PDC 比较，70 个 miRNA 差异表达（log FC≥1，padj<0.05）。三个比较中上调基因主要参与生物过程对刺激的反应，而下调基因主要参与神经系统发育（p<.01）。PI 与 PD 和 PI 与 PIC 比较上调基因均参与 Toll 样受体信号和 RIG-I 样信号。PI 与 PD 比较上调基因参与 T 和 B 细胞受体信号、细胞凋亡和破骨细胞分化。三个比较中粘着斑均下调，PI 与 PD 和 PD 与 PDC 比较粘着连接也下调（p<.1）。
本研究显示种植体周围炎和牙周炎之间 miRNA 表达谱存在差异，并注释了可能的靶基因和分子机制；可为制定管理策略奠定基础。
