Department of Periodontology, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Republic of Korea; Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Republic of Korea.
Biomedical Knowledge Engineering Laboratory, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Republic of Korea.
Gene. 2020 Feb 15;727:144258. doi: 10.1016/j.gene.2019.144258. Epub 2019 Nov 21.
Peri-implantitis is similar to periodontitis in both symptoms and treatment; however, their level of similarity remains controversial. Here, we compared multiple cases of periodontitis and peri-implantitis through transcriptome and methylome profiling, and analyzed the effects of smoking as a typical risk factor. Human gingival tissues were obtained from 20 patients with periodontitis or peri-implantitis via periodontal surgical procedures. Total RNA and genomic DNA were isolated, and transcriptome and methylome datasets were generated. Comprehensive analysis of differential gene expression, DNA methylation, and protein-protein interactions indicated that periodontitis and peri-implantitis share biological similarities; however, hierarchical clustering between the two disease groups revealed distinct molecular characteristics. These differences might be related to structural differences in natural tooth-bone and implant-bone. Additionally, smoking differentially affected periodontitis and peri-implantitis in terms of host-defense mechanism impairment. Within the limitations of this study, the results provide evidence that peri-implantitis is distinct from periodontitis and that smoking potentially affects disease progression. Our study provides a foundation for the rational design of a large-scale study in the future for a more comprehensive analysis that includes microbiome and clinical data.
种植体周围炎在症状和治疗上与牙周炎相似，但它们的相似程度仍存在争议。在这里，我们通过转录组和甲基化组分析比较了多个牙周炎和种植体周围炎病例，并分析了吸烟作为典型危险因素的影响。通过牙周手术从 20 名牙周炎或种植体周围炎患者中获得人牙龈组织。分离总 RNA 和基因组 DNA，并生成转录组和甲基化组数据集。对差异基因表达、DNA 甲基化和蛋白质-蛋白质相互作用的综合分析表明，牙周炎和种植体周围炎具有生物学相似性；然而，两组疾病之间的层次聚类显示出明显的分子特征。这些差异可能与天然牙-骨和种植体-骨的结构差异有关。此外，吸烟在宿主防御机制受损方面对牙周炎和种植体周围炎的影响存在差异。在本研究的限制范围内，结果表明种植体周围炎与牙周炎不同，吸烟可能影响疾病进展。我们的研究为未来更全面的分析提供了基础，包括微生物组和临床数据的合理设计。
