Chemistry Department, Faculty of Science, South Valley University, Qena 83523, Egypt.
Chemistry Department, Faculty of Science, South Valley University, Qena 83523, Egypt.
Int J Biol Macromol. 2022 Oct 31;219:1062-1079. doi: 10.1016/j.ijbiomac.2022.07.249. Epub 2022 Aug 6.
In this paper, chitosan (Chi) was successfully reacted with natural mono ketone namely, camphor (Cam), through a condensation reaction to obtain a novel Chi-Cam polymer. The encapsulation of Chi-Cam polymer into the cavity of β-cyclodextrin biopolymer by inclusion complex method, and the immobilization of TiO NPs into Chi-Cam matrix by metal complex method were performed. A set of characterizations (XRD, FTIR, SEM, UV-Vis., optical band gap, Urbach energy, refractive index, and thermogravimetric analysis) were implemented to incorporate their properties with applications. It was not observed the existence of any strange peaks in diffractogram patterns of all prepared samples, confirming the proper preparation and high purity polymers. The crystalline index was found to be 69.41, 41.58, 58.25 and 44.2 % for Chi, Chi-Cam, polyrotaxane, and nanocomposite, respectively. High surface area, lowest pores size, and lowest pores volume were confirmed the micropores of composite, which also could provide more reactive sites, and enhancing the bioactivity. The tested Chi-Cam/TiO NPs possessed the highest antimicrobial impact, followed by the Chi-Cam/β-CD towards pathogenic microbes. The highest % inhibition of biofilm formation by Chi-Cam, Chi-Cam/β-CD, and Chi-Cam/TiO NPs was noted for S. aureus (40.05 %, 80.21 %, and 87.49 %), E. coli (38.56 %, 74.32 % and 83.32 %), P. aureginosa (32.12 %, 58.80 %, and 74.53 %), and C. albicans (13.69 %, 27.89 % and 70.89 %), respectively. The obtaining findings pave the route towards useful utilization of potential materials for biomedical applications.
本文通过缩合反应成功地将壳聚糖（Chi）与天然单酮即莰酮（Cam）反应，得到了一种新型 Chi-Cam 聚合物。通过包合络合法将 Chi-Cam 聚合物包封到β-环糊精生物聚合物的空腔中，并通过金属络合法将 TiO NPs 固定到 Chi-Cam 基质中。进行了一系列的特性分析（XRD、FTIR、SEM、UV-Vis、光学带隙、Urbach 能、折射率和热重分析），以将其性质与应用相结合。在所有制备样品的衍射图谱中都没有观察到任何奇异峰的存在，这证实了制备方法正确且聚合物纯度高。Chi、Chi-Cam、聚轮烷和纳米复合材料的结晶指数分别为 69.41%、41.58%、58.25%和 44.2%。高比表面积、最低孔径和最低孔体积证实了复合材料的微孔，这也可以提供更多的反应位点，并增强生物活性。测试的 Chi-Cam/TiO NPs 具有最高的抗菌效果，其次是 Chi-Cam/β-CD 对病原微生物。Chi-Cam、Chi-Cam/β-CD 和 Chi-Cam/TiO NPs 对金黄色葡萄球菌（40.05%、80.21%和 87.49%）、大肠杆菌（38.56%、74.32%和 83.32%）、铜绿假单胞菌（32.12%、58.80%和 74.53%）和白色念珠菌（13.69%、27.89%和 70.89%）的生物膜形成抑制率最高。研究结果为生物医学应用的潜在材料的有用利用铺平了道路。
