Department of Chemistry, Faculty of Science and Arts, Najran University, Najran, Saudi Arabia.
Department of Chemistry, College of Science, Qassim University, Buraidah 51452, Saudi Arabia; Department of Chemistry, Faculty of Science, Cairo University, Giza 12613, Egypt.
Int J Biol Macromol. 2022 Apr 30;205:719-730. doi: 10.1016/j.ijbiomac.2022.03.076. Epub 2022 Mar 17.
Two novel chitosan derivatives were prepared by incorporating salicylhydrazide into chitosan Schiff base (SCsSB) and chitosan (SCs). Two nanocomposites, SCs/TiO-1% and SCs/TiO-3%, were also prepared. Their structures were confirmed using elemental analyses, FTIR, XRD, SEM, EDX and TEM. Their antimicrobial and anti-biofilm activities were arranged as: SCs/TiO-3% > SCs/TiO-1% > SCs > SCsSB > chitosan. SCs showed minimum inhibitory concentration (MIC) value of 1.95 μg/mL against A. niger which was comparable with that of Amphotericin B. SCs/TiO-3% showed higher inhibition against S. epidermidis, S. aureus, S. pyogenes, P. aeruginosa and E. coli than Vancomycin. While, it showed comparable inhibition activity to that of Vancomycin against B. subtilis and P. mirabilis. SCs/TiO-3% showed MIC values equal 0.48 and 0.98 μg/mL corresponded to 0.98 and 1.95 μg/mL of Amphotericin B against C. albicans, A. fumigatus and A. niger, respectively. SCs/TiO-3% showed much lower minimum biofilm inhibitory concentration (MBIC) values, ranged between 1.95 and 7.81 μg/mL, than those of SCs, ranged from 62.5 to 125 μg/mL. SCs/TiO-3% was safe on normal human cells. The modifiers and TiO nanoparticles incorporated into chitosan in one structure developed its performance. It is approach for attaining appropriate structures which are good competitors for antimicrobial agents.
两种新型壳聚糖衍生物通过将水杨醛腙掺入壳聚糖席夫碱（SCsSB）和壳聚糖（SCs）中制备。还制备了两种纳米复合材料，SCs/TiO-1%和SCs/TiO-3%。通过元素分析、FTIR、XRD、SEM、EDX 和 TEM 确认它们的结构。它们的抗菌和抗生物膜活性排列如下：SCs/TiO-3%>SCs/TiO-1%>SCs>SCsSB>壳聚糖。壳聚糖对黑曲霉的最小抑菌浓度（MIC）值为 1.95μg/mL，与两性霉素 B 相当。SCs/TiO-3%对表皮葡萄球菌、金黄色葡萄球菌、化脓性链球菌、铜绿假单胞菌和大肠杆菌的抑制作用高于万古霉素。然而，它对枯草芽孢杆菌和奇异变形杆菌的抑制活性与万古霉素相当。SCs/TiO-3%对白色念珠菌、烟曲霉和黑曲霉的 MIC 值分别为 0.48 和 0.98μg/mL，分别相当于两性霉素 B 的 0.98 和 1.95μg/mL。SCs/TiO-3%的最低生物膜抑制浓度（MBIC）值为 1.95-7.81μg/mL，远低于壳聚糖的 62.5-125μg/mL。SCs/TiO-3%对正常人体细胞安全。修饰剂和 TiO 纳米粒子在一个结构中掺入壳聚糖，从而提高了其性能。这是获得具有良好抗菌性能的适当结构的一种方法。
