Identification of cells expressing T and p28,33 (Ia-like) antigens in sections of human lymphoid tissue.

A method has been developed for identifying human lymphocyte populations in tissue sections using hetero-antisera to T cells and to the B cell associated Ia-like, p28,33 polypeptide complex. The differential topographical localisation of T and B cells is revealed by simultaneous staining with antibodies tagged with different fluorochromes; this approach also identifies a small population of T cells bearing Ia-like antigens. Preliminary studies on lymphomatous tissue suggests this approach will be of value in the study of malignancy and cellular infiltration in disease.