Liu Dandan, Chen Yidong, Ren Yixin, Yuan Peng, Wang Nan, Liu Qiang, Yang Cen, Yan Zhiqiang, Yang Ming, Wang Jing, Lian Ying, Yan Jie, Zhai Fan, Nie Yanli, Zhu Xiaohui, Chen Yuan, Li Rong, Chang Hsun-Ming, Leung Peter C K, Qiao Jie, Yan Liying
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.
National Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China.
Sci Adv. 2022 Aug 12;8(32):eabj3725. doi: 10.1126/sciadv.abj3725. Epub 2022 Aug 10.
Mechanisms of implantation such as determination of the attachment pole, fetal-maternal communication, and underlying causes of implantation failure are largely unexplored. Here, we performed single-cell RNA sequencing on peri-implantation embryos from both humans and mice to explore trophectoderm (TE) development and embryo-endometrium cross-talk. We found that the transcriptomes of polar and mural TE diverged after embryos hatched from the zona pellucida in both species, with polar TE being more mature than mural TE. The implantation poles show similarities in cell cycle activities, as well as in expression of genes critical for implantation and placentation. Embryos that either fail to attach in vitro or fail to implant in vivo show abnormalities in pathways related to energy production, protein metabolism, and 18 ribosomal RNA mA methylation. These findings uncover the gene expression characteristics of humans and mice TE differentiation during the peri-implantation period and provide new insights into embryo implantation.
诸如着床附着极的确定、胎儿与母体的交流以及着床失败的潜在原因等着床机制在很大程度上尚未得到充分探索。在此，我们对人类和小鼠着床期胚胎进行了单细胞RNA测序，以探究滋养外胚层（TE）的发育以及胚胎与子宫内膜的相互作用。我们发现，在两个物种中，胚胎从透明带孵化后，极滋养外胚层（polar TE）和壁滋养外胚层（mural TE）的转录组出现分化，极滋养外胚层比壁滋养外胚层更成熟。着床极在细胞周期活动以及对着床和胎盘形成至关重要的基因表达方面表现出相似性。在体外无法附着或在体内无法着床的胚胎在与能量产生、蛋白质代谢和18核糖体RNA mA甲基化相关的途径中表现出异常。这些发现揭示了人类和小鼠在着床期TE分化的基因表达特征，并为胚胎着床提供了新的见解。
