Flick J A, Schnaar R L, Perman J A
Clin Chem. 1987 Jul;33(7):1211-2.
Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detected with high reproducibility and without interference by urinary chromogens. After oral administration, the urinary excretion of lactulose in 8 h equaled 2.33 (SD 1.86)% in 15 patients with cystic fibrosis, as compared with 0.13 (SD 0.12)% in 16 healthy subjects (P less than 0.001).
口服乳果糖后的尿排泄情况被用作肠道通透性的指标。我们开发了一种简单的薄层色谱技术来测定尿液中的乳果糖，使用硅胶60板和丙醇 - 硼酸盐溶剂系统。低至62.5mg/L的乳果糖浓度能够被高重复性地检测到，且不受尿色素原的干扰。口服后，15名囊性纤维化患者在8小时内乳果糖的尿排泄量为2.33（标准差1.86）%，而16名健康受试者为0.13（标准差0.12）%（P<0.001）。
