• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

的靶向诱变可稳定马铃薯细胞培养物中花青素的产生。 （原文中“Targeted mutagenesis of”后面似乎缺少具体内容）

Targeted mutagenesis of stabilizes the production of anthocyanins in potato cell culture.

作者信息

D'Amelia Vincenzo, Staiti Annalisa, D'Orso Fabio, Maisto Maria, Piccolo Vincenzo, Aversano Riccardo, Carputo Domenico

机构信息

Institute of Biosciences and Bioresources (IBBR) National Research Council of Italy Portici Italy.

Department of Agricultural Sciences University of Naples Federico II Portici Italy.

出版信息

Plant Direct. 2022 Aug 4;6(8):e433. doi: 10.1002/pld3.433. eCollection 2022 Aug.

DOI:10.1002/pld3.433
PMID:35949953
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9352536/
Abstract

To increase the production of decorated anthocyanins in potato cell cultures, we knocked out a novel potato gene, named (), using CRISPR-Cas9 editing. Our results provided evidence that mutant cell lines doubled the accumulation level of anthocyanins biosynthesized. Moreover, the production of these important pigments was stabilized over time. Our study overcame important challenges in the efficient biotechnological production of these valuable pigments and reported the function of a novel anthocyanin biosynthesis repressor gene.

摘要

为了提高马铃薯细胞培养物中修饰花青素的产量，我们使用CRISPR-Cas9编辑技术敲除了一个名为（）的新马铃薯基因。我们的结果表明，突变细胞系中生物合成的花青素积累水平增加了一倍。此外，这些重要色素的产量随时间推移保持稳定。我们的研究克服了这些有价值色素高效生物技术生产中的重大挑战，并报道了一种新的花青素生物合成抑制基因的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/479a/9352536/3aff29ea5ec2/PLD3-6-e433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/479a/9352536/3aff29ea5ec2/PLD3-6-e433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/479a/9352536/3aff29ea5ec2/PLD3-6-e433-g002.jpg

相似文献

1
Targeted mutagenesis of stabilizes the production of anthocyanins in potato cell culture.的靶向诱变可稳定马铃薯细胞培养物中花青素的产生。 （原文中“Targeted mutagenesis of”后面似乎缺少具体内容）
Plant Direct. 2022 Aug 4;6(8):e433. doi: 10.1002/pld3.433. eCollection 2022 Aug.
2
Genetic and epigenetic dynamics affecting anthocyanin biosynthesis in potato cell culture.影响马铃薯细胞培养中花青素生物合成的遗传和表观遗传动力学
Plant Sci. 2020 Sep;298:110597. doi: 10.1016/j.plantsci.2020.110597. Epub 2020 Jul 9.
3
Functional diversification of the potato R2R3 MYB anthocyanin activators AN1, MYBA1, and MYB113 and their interaction with basic helix-loop-helix cofactors.马铃薯R2R3 MYB花青素激活因子AN1、MYBA1和MYB113的功能多样性及其与碱性螺旋-环-螺旋辅助因子的相互作用。
J Exp Bot. 2016 Apr;67(8):2159-76. doi: 10.1093/jxb/erw014. Epub 2016 Feb 16.
4
Characterization of the Locus Responsible for Floral Anthocyanin Production in Potato.马铃薯中负责花色素苷生成的基因座的特征分析
G3 (Bethesda). 2020 Oct 5;10(10):3871-3879. doi: 10.1534/g3.120.401684.
5
Genome Editing in Potato with CRISPR/Cas9.利用CRISPR/Cas9对马铃薯进行基因组编辑
Methods Mol Biol. 2019;1917:183-201. doi: 10.1007/978-1-4939-8991-1_14.
6
CRISPR/Cas9-mediated SlAN2 mutants reveal various regulatory models of anthocyanin biosynthesis in tomato plant.CRISPR/Cas9 介导的 SlAN2 突变体揭示了番茄植物中花色苷生物合成的各种调控模式。
Plant Cell Rep. 2020 Jun;39(6):799-809. doi: 10.1007/s00299-020-02531-1. Epub 2020 Mar 27.
7
Genetic control of anthocyanin pigmentation of potato tissues.马铃薯组织花青素色素沉着的遗传控制
BMC Genet. 2019 Mar 18;20(Suppl 1):27. doi: 10.1186/s12863-019-0728-x.
8
StWRKY13 promotes anthocyanin biosynthesis in potato (Solanum tuberosum) tubers.StWRKY13 促进马铃薯（Solanum tuberosum）块茎中的花色素苷生物合成。
Funct Plant Biol. 2021 Dec;49(1):102-114. doi: 10.1071/FP21109.
9
Metabolite profiling of red and blue potatoes revealed cultivar and tissue specific patterns for anthocyanins and other polyphenols.红皮土豆和蓝皮土豆的代谢物谱分析揭示了花青素和其他多酚类物质的品种及组织特异性模式。
Planta. 2017 Aug;246(2):281-297. doi: 10.1007/s00425-017-2718-4. Epub 2017 Jun 29.
10
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato.利用 CRISPR/Cas9 介导的多位点向导 RNA 靶向诱变技术在马铃薯中的应用。
Transgenic Res. 2023 Oct;32(5):383-397. doi: 10.1007/s11248-023-00356-8. Epub 2023 Jun 18.

引用本文的文献

1
Unlocking Opportunities and Overcoming Challenges in Genetically Engineered Biofortification.解锁基因工程生物强化中的机遇并克服挑战。
Nutrients. 2025 Jan 30;17(3):518. doi: 10.3390/nu17030518.
2
Accumulation of Anthocyanin in the Aleurone of Barley Grains by Targeted Restoration of the Gene.通过基因的靶向恢复使花青素在大麦籽粒糊粉层中积累。
Int J Mol Sci. 2024 Nov 26;25(23):12705. doi: 10.3390/ijms252312705.
3
Integrative analysis of metabolome and transcriptome reveals a dynamic regulatory network of potato tuber pigmentation.

本文引用的文献

1
CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives.马铃薯中的CRISPR/Cas基因组编辑：现状与未来展望
Front Genet. 2022 Feb 2;13:827808. doi: 10.3389/fgene.2022.827808. eCollection 2022.
2
Repressors of anthocyanin biosynthesis.花色素苷生物合成的抑制剂。
New Phytol. 2021 Aug;231(3):933-949. doi: 10.1111/nph.17397. Epub 2021 May 28.
3
Genetic and epigenetic dynamics affecting anthocyanin biosynthesis in potato cell culture.影响马铃薯细胞培养中花青素生物合成的遗传和表观遗传动力学
代谢组和转录组的综合分析揭示了马铃薯块茎色素沉着的动态调控网络。
iScience. 2022 Dec 29;26(2):105903. doi: 10.1016/j.isci.2022.105903. eCollection 2023 Feb 17.
Plant Sci. 2020 Sep;298:110597. doi: 10.1016/j.plantsci.2020.110597. Epub 2020 Jul 9.
4
Anthocyanin Fruit encodes an R2R3-MYB transcription factor, SlAN2-like, activating the transcription of SlMYBATV to fine-tune anthocyanin content in tomato fruit.花青素水果编码一种R2R3-MYB转录因子，类似SlAN2，激活SlMYBATV的转录以微调番茄果实中的花青素含量。
New Phytol. 2020 Mar;225(5):2048-2063. doi: 10.1111/nph.16272. Epub 2019 Nov 21.
5
MYB Repressors as Regulators of Phenylpropanoid Metabolism in Plants.MYB 抑制剂作为植物苯丙烷代谢的调节剂。
Trends Plant Sci. 2019 Mar;24(3):275-289. doi: 10.1016/j.tplants.2018.12.003. Epub 2019 Jan 29.
6
Colour bio-factories: Towards scale-up production of anthocyanins in plant cell cultures.彩色生物工厂：在植物细胞培养中大规模生产花青素。
Metab Eng. 2018 Jul;48:218-232. doi: 10.1016/j.ymben.2018.06.004. Epub 2018 Jun 8.
7
A putative R3 MYB repressor is the candidate gene underlying atroviolacium, a locus for anthocyanin pigmentation in tomato fruit.一个假定的 R3 MYB 阻遏物是位于番茄果实花青素色素形成的 atroviolacium 基因座的候选基因。
J Exp Bot. 2017 Dec 16;68(21-22):5745-5758. doi: 10.1093/jxb/erx382.
8
Metabolite profiling of red and blue potatoes revealed cultivar and tissue specific patterns for anthocyanins and other polyphenols.红皮土豆和蓝皮土豆的代谢物谱分析揭示了花青素和其他多酚类物质的品种及组织特异性模式。
Planta. 2017 Aug;246(2):281-297. doi: 10.1007/s00425-017-2718-4. Epub 2017 Jun 29.
9
Stability-increasing effects of anthocyanin glycosyl acylation.花色苷糖基酰化的稳定性增强作用。
Food Chem. 2017 Jan 1;214:119-128. doi: 10.1016/j.foodchem.2016.07.073. Epub 2016 Jul 11.
10
CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing.CRISP-ID：通过 Sanger 测序解码 CRISPR 介导的插入缺失。
Sci Rep. 2016 Jul 1;6:28973. doi: 10.1038/srep28973.