Lee Man Bo, Shekasteband Reza, Hutton Samuel F, Lee Tong Geon
Gulf Coast Research and Education Center University of Florida Wimauma Florida USA.
Department of Horticultural Science North Carolina State University, Mountain Horticultural Crops Research & Extension Center Mills River North Carolina USA.
Plant Direct. 2022 Aug 4;6(8):e422. doi: 10.1002/pld3.422. eCollection 2022 Aug.
Reduced plant height due to shortened stems is beneficial for improving crop yield potential, better resilience to biotic/abiotic stresses, and rapid crop producer adoption of the agronomic and management practices. Breeding tomato plants with a reduced height, however, poses a particular challenge because this trait is often associated with a significant fruit size (weight) reduction. The tomato locus controls plant height. Genetic mapping and genome assembly revealed three () genes located within the mapping interval, and a complete coding sequence deletion of the telomere proximal () was found in the allele but not in . The knock-out of in large-fruited fresh-market tomato reduced the height and fruit yield, but the ability to produce large size fruits was retained. However, concurrent yield evaluation of a pair of sister lines with or without the allele revealed that artificial selection contributes to commercially acceptable yield potential in tomatoes. A network analysis of gene-expression patterns across genotypes, tissues, and the gibberellic acid (GA) treatment revealed that member(s) of the family may play a role in the suppression of the GA biosynthesis in roots and provided a framework for identifying the responsible molecular signaling pathways in -mediated phenotypic changes. Lastly, mutations of homologs also resulted in reduced height. These results shed light on the genetic and physiological mechanisms by which the allele alters tomato architecture.
由于茎缩短导致的植株高度降低有利于提高作物产量潜力、增强对生物/非生物胁迫的抵抗力，以及使作物生产者迅速采用农艺和管理措施。然而，培育矮化番茄植株面临着特殊挑战，因为这一性状通常与果实大小（重量）显著降低相关。番茄 基因座控制植株高度。遗传图谱绘制和基因组组装揭示了位于 定位区间内的三个 基因，并且在 等位基因中发现端粒近端 的完整编码序列缺失，而在 中未发现。在大果型鲜食番茄中敲除 会降低植株高度和果实产量，但保留了产生大果实的能力。然而，对一对具有或不具有 等位基因的姊妹系进行的产量评估表明，人工选择有助于 番茄获得商业上可接受的产量潜力。对不同基因型、组织和赤霉素（GA）处理的基因表达模式进行网络分析发现， 家族成员可能在抑制根中的GA生物合成中发挥作用，并为确定 -介导的表型变化中的相关分子信号通路提供了框架。最后， 同源基因的突变也导致植株高度降低。这些结果揭示了 等位基因改变番茄株型的遗传和生理机制。
