Department of Dentistry, National Taiwan University Hospital and Graduate Institute of Clinical Dentistry, National Taiwan University, Taipei, Taiwan.
Department of Neurology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.
Oral Dis. 2023 Nov;29(8):3677-3687. doi: 10.1111/odi.14348. Epub 2022 Aug 30.
To investigate the association between Alzheimer's disease (AD) and periodontitis in the aspects of periodontal status, serological markers, and oral microbiome.
Twenty AD and 20 healthy subjects were enrolled in this age- and gender-matched case-control study. Clinical periodontal parameters and serum biomarkers, including amyloid β (Aβ ), Tau, phosphorylated Tau (pTau), triglyceride, pro-inflammatory cytokines, and anti-Porphyromonas gingivalis lipopolysaccharide (LPS) antibody were examined. The saliva samples were analyzed for oral microbiome composition.
Alzheimer's disease patients with Clinical Dementia Rating (CDR) ≥1 exhibited significantly more clinical attachment loss (CAL) than those with lower CDR. The levels of serum Tau protein, hsCRP and anti-P. gingivalis LPS antibody were markedly elevated in the AD group compared with the control group. Serum pTau protein level was positively correlated with anti-P. gingivalis LPS antibody titer. Moreover, the increased abundances of Capnocytophaga sp ora clone DZ074, Eubacterium infirmum, Prevotella buccae, and Selenomonas artemidis were detected in the AD group. Interestingly, serum levels of Aβ pTau, and anti-P. gingivalis LPS antibody were strongly related to the gene upregulation in human pathogen septicemia.
Our study suggested the association of periodontal infection and oral microbiome with AD. Further large-scale studies with longitudinal follow-up are warranted.
从牙周状况、血清标志物和口腔微生物组学方面，探讨阿尔茨海默病（AD）与牙周炎的关系。
本项年龄和性别匹配的病例对照研究纳入了 20 例 AD 患者和 20 例健康对照者。检测了临床牙周参数和血清生物标志物，包括淀粉样β（Aβ）、Tau 蛋白、磷酸化 Tau 蛋白（pTau）、甘油三酯、促炎细胞因子和抗牙龈卟啉单胞菌脂多糖（LPS）抗体。分析了唾液样本中的口腔微生物组组成。
与 CDR 较低的 AD 患者相比，CDR≥1 的 AD 患者的临床附着丧失（CAL）明显更多。与对照组相比，AD 组的 Tau 蛋白、hsCRP 和抗牙龈卟啉单胞菌 LPS 抗体水平明显升高。血清 pTau 蛋白水平与抗牙龈卟啉单胞菌 LPS 抗体滴度呈正相关。此外，AD 组还检测到 Capnocytophaga sp ora clone DZ074、Eubacterium infirmum、Prevotella buccae 和 Selenomonas artemidis 的丰度增加。有趣的是，Aβ、pTau 和抗牙龈卟啉单胞菌 LPS 抗体的血清水平与人类病原体败血症的基因上调密切相关。
本研究提示牙周感染和口腔微生物组与 AD 有关。需要进一步进行大规模、纵向随访的研究。
