School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Institute of Wheat Research, Shanxi Agricultural University/State Key Laboratory of Sustainable Dryland Agriculture, Taiyuan, 030031, China.
Theor Appl Genet. 2022 Sep;135(9):3127-3141. doi: 10.1007/s00122-022-04175-z. Epub 2022 Aug 11.
Genetic architecture controlling grain lutein content of common wheat was investigated through an integration of genome-wide association study (GWAS) and linkage analysis. Putative candidate genes involved in carotenoid metabolism and regulation were identified, which provide a basis for gene cloning and development of nutrient-enriched wheat varieties through molecular breeding. Lutein, known as 'the eye vitamin', is an important component of wheat nutritional and end-use quality. However, the genetic manipulation of grain lutein content (LUC) in common wheat has not previously been well studied. Here, quantitative trait loci (QTL) associated with the LUC measured by high performance liquid chromatography (HPLC) were first identified by integrating a genome-wide association study (GWAS) and linkage mapping. A Chinese wheat mini-core collection (MCC) of 262 accessions and a doubled haploid (DH) population derived from Jinchun 7 and L1219 were genotyped using the 90K SNP array. A total of 124 significant marker-trait associations (MTAs) on all 21 wheat chromosomes except for 1A, 4D, and 5B that formed 58 QTL were detected. Among them, six stable QTL were identified on chromosomes 2AL, 2DS, 3BL, 3DL, 7AL, and 7BS. Meanwhile, three of the ten QTL identified in the DH population, QLuc.5A.1 and QLuc.5A.2 on chromosome 5AL and QLuc.6A.2 on 6AS, were stable and independently explained 5.58-10.86% of the phenotypic variation. The QLuc.6A.2 region colocalized with two MTAs identified by GWAS. Moreover, 71 carotenoid metabolism-related candidate genes were identified, and the allelic effects were analyzed in the MCC panel based on the 90K array. Results revealed that the genes CYP97A3 (Chr. 6B) and CCD1 (Chr. 5A) were significantly associated with LUC. Additionally, the gene PSY3 (QLuc.5A.1) and several candidate genes involved in the methylerythritol 4-phosphate (MEP) pathways colocalized with stable QTL regions. The present study provides potential targets for future functional gene exploration and molecular breeding in common wheat.
通过整合全基因组关联研究（GWAS）和连锁分析，研究了控制普通小麦叶黄素含量的遗传结构。鉴定了参与类胡萝卜素代谢和调控的假定候选基因，为通过分子育种进行富含营养的小麦品种的基因克隆和开发提供了基础。叶黄素，俗称“眼睛维生素”，是小麦营养和用途品质的重要组成部分。然而，普通小麦中粒叶黄素含量（LUC）的遗传操作以前尚未得到很好的研究。在这里，通过整合全基因组关联研究（GWAS）和连锁作图，首次鉴定了与高效液相色谱（HPLC）测量的 LUC 相关的数量性状位点（QTL）。使用 90K SNP 阵列对来自金春 7 号和 L1219 的中国小麦微核心集合（MCC）262 个品系和双单倍体（DH）群体进行了基因型分析。在除 1A、4D 和 5B 之外的 21 条小麦染色体上共检测到 124 个显著的标记-性状关联（MTAs），形成了 58 个 QTL。其中，在 2AL、2DS、3BL、3DL、7AL 和 7BS 染色体上鉴定出 6 个稳定的 QTL。同时，在 DH 群体中鉴定出的 10 个 QTL 中有 3 个稳定，独立解释了 5.58-10.86%的表型变异，分别位于 5AL 上的 QLuc.5A.1 和 QLuc.5A.2 以及 6AS 上的 QLuc.6A.2。QLuc.6A.2 区域与通过 GWAS 鉴定的两个 MTAs 共定位。此外，在 90K 阵列的 MCC 面板上分析了 71 个与类胡萝卜素代谢相关的候选基因的等位效应。结果表明，基因 CYP97A3（Chr.6B）和 CCD1（Chr.5A）与 LUC 显著相关。此外，基因 PSY3（QLuc.5A.1）和参与甲基赤藓醇 4-磷酸（MEP）途径的几个候选基因与稳定的 QTL 区域共定位。本研究为普通小麦未来的功能基因探索和分子育种提供了潜在的目标。
