Key Laboratory of Evidence Identification in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, Shandong 250014, People's Republic of China.
Institute of Forensic Pathology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, People's Republic of China.
Leg Med (Tokyo). 2022 Nov;59:102132. doi: 10.1016/j.legalmed.2022.102132. Epub 2022 Aug 6.
Species identification of unknown biological samples is crucial for forensic applications, especially in cases of explosion, disaster accidents, and body mutilation after murdering, as well as poaching, illegal trade in endangered animals, and meat food fraud. In this study, we identified 60 volatile organic compounds (VOCs) in fresh skeletal muscle tissues of seven different animal species (cattle, sheep, pigs, rabbits, rats, chickens and carp) and a human dead body by headspace-gas-chromatography ion-mobility spectrometry (HS-GC-IMS), and compared their differences by retention time, drift time and molecular weight. The results showed that these VOCs formed different gallery plot fingerprints in the skeletal muscle tissues of the human dead body and seven animal species. Principal component analysis (PCA) showed significantly different fingerprints between these species, and these fingerprints maintained good stability between the species and within the same species. Some VOCs have high species specificity, while VOCs of human fresh muscle tissues from different individual sources have little difference, demonstrating that all tested muscle tissue samples could be distinguished based on different VOCs. HS-GC-IMS has proved to be a rapid, high-throughput, highly sensitive and specific species identification method, which can be used for forensic species identification in criminal cases and disaster accidents, as well as detection in the field of food safety, such as meat fraud and adulteration.
物种鉴定在法医学领域至关重要，特别是在爆炸、灾难事故、碎尸以及非法捕猎、濒危动物非法交易、肉类食品欺诈等案件中。本研究通过顶空-气相色谱离子迁移谱法（HS-GC-IMS），对来自 7 种不同动物（牛、羊、猪、兔、鼠、鸡和鲤鱼）和 1 具人类尸体的新鲜骨骼肌组织中的 60 种挥发性有机化合物（VOCs）进行了鉴定，并通过保留时间、漂移时间和分子量对其进行了比较。结果表明，这些 VOCs 在人类尸体和 7 种动物骨骼肌组织中形成了不同的图库指纹。主成分分析（PCA）表明，这些物种之间的指纹图谱存在显著差异，并且这些指纹图谱在物种之间和同一物种内具有良好的稳定性。一些 VOCs 具有很高的物种特异性，而不同个体来源的人类新鲜肌肉组织中的 VOCs 差异很小，表明所有测试的肌肉组织样本都可以基于不同的 VOCs 进行区分。HS-GC-IMS 已被证明是一种快速、高通量、高灵敏度和特异性的物种鉴定方法，可用于刑事案件和灾难事故中的法医物种鉴定，以及食品安全领域（如肉类欺诈和掺假）的检测。
