微小 RNA-375 通过靶向 CST1 抑制喉鳞状细胞癌进展。
MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting CST1.
机构信息
Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Pingxiang, China.
Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and Neck Surgery, Pingxiang, China.
出版信息
Braz J Otorhinolaryngol. 2022 Nov-Dec;88 Suppl 4(Suppl 4):S108-S116. doi: 10.1016/j.bjorl.2022.06.005. Epub 2022 Jul 6.
OBJECTIVE
This study aims to explore the effect and mechanism of miR-375 in Laryngeal Squamous Cell Carcinoma (LSCC) cell progression.
METHODS
LSCC cells (LSC-1 and TU177) were transfected with miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1. The expression of miR-375, CST1, MMP-2, and MMP-9 was measured. The effect of miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1 on cell biological functions, including cell proliferation, migration, invasion, and apoptosis, was also assessed. The potential relationship between CST1 and miR-375 was predicted by Jefferson software and validated by dual luciferase reporter gene assay.
RESULTS
Downregulated miR-375 expression was found in LSCC cells. Overexpression of miR-375 inhibited the viability and migration and promoted apoptosis of LSCC cells. Jefferson database and dual luciferase reporter gene assay confirmed that miR-375 directly targeted CST1. Overexpression of CST1 could reverse the anti-cancer effect of miR-375 overexpression in LSCC cells.
CONCLUSION
Collected evidence showed that miR-375/CST1 axis was implicated in LSCC progression.
LEVEL OF EVIDENCE
Level 3.
目的
本研究旨在探讨 miR-375 在喉鳞状细胞癌（LSCC）细胞进展中的作用和机制。
方法
转染 LSCC 细胞（LSC-1 和 TU177）miR-375-模拟物、miR-375-抑制剂或 miR-375-模拟物+过表达-CST1。测量 miR-375、CST1、MMP-2 和 MMP-9 的表达。还评估了 miR-375-模拟物、miR-375-抑制剂或 miR-375-模拟物+过表达-CST1 对细胞生物学功能的影响，包括细胞增殖、迁移、侵袭和凋亡。Jefferson 软件预测 CST1 和 miR-375 之间的潜在关系，并通过双荧光素酶报告基因检测进行验证。
结果
在 LSCC 细胞中发现 miR-375 表达下调。miR-375 的过表达抑制了 LSCC 细胞的活力和迁移，并促进了细胞凋亡。Jefferson 数据库和双荧光素酶报告基因检测证实 miR-375 可直接靶向 CST1。CST1 的过表达可逆转 miR-375 过表达对 LSCC 细胞的抗癌作用。
结论
研究结果表明，miR-375/CST1 轴参与了 LSCC 的进展。
证据水平
3 级。
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