Minneapolis Veterans Affairs Health Care System, Minneapolis, MN, USA.
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA.
BMC Res Notes. 2022 Aug 11;15(1):275. doi: 10.1186/s13104-022-06165-2.
Chronic multisymptom illness (CMI) is an idiopathic disease affecting thousands of U.S. Veterans exposed to open-air burn pits emitting aerosolized particulate matter (PM) while serving in Central and Southwest Asia and Africa. Exposure to burn pit PM can result in profound biologic consequences including chronic fatigue, impaired cognition, and respiratory diseases. Dysregulated or unresolved inflammation is a possible underlying mechanism for CMI onset. We describe a rat model of whole-body inhalation exposure using carbon black nanoparticles (CB) as a surrogate for military burn pit-related exposure. Using this model, we measured biomarkers of inflammation in multiple tissues.
Male Sprague Dawley rats were exposed to CB aerosols by whole body inhalation (6 ± 0.83 mg/m). Proinflammatory biomarkers were measured in multiple tissues including arteries, brain, lung, and plasma. Biomarkers of cardiovascular injury were also assayed in plasma. CB inhalation exposure increased CMI-related proinflammatory biomarkers such as IFN-γ and TNFα in multiple tissue samples. CB exposure also induced cardiovascular injury markers (adiponectin, MCP1, sE-Selectin, sICam-1 and TIMP1) in plasma. These findings support the validity of our animal exposure model for studies of burn pit-induced CMI. Future studies will model more complex toxicant mixtures as documented at multiple burn pit sites.
慢性多症状疾病（CMI）是一种特发性疾病，影响了成千上万的美国退伍军人，他们在中 央和西南亚以及非洲服役时接触到露天燃烧坑，燃烧坑会排放气溶胶颗粒（PM）。暴露于燃烧坑 PM 会导致严重的生物学后果，包括慢性疲劳、认知障碍和呼吸道疾病。炎症失调或未解决可能是 CMI 发病的潜在机制。我们描述了一种使用碳黑纳米颗粒（CB）作为军事燃烧坑相关暴露替代物的全身吸入暴露大鼠模型。使用该模型，我们测量了多个组织中的炎症生物标志物。
雄性 Sprague Dawley 大鼠通过全身吸入（6±0.83mg/m）暴露于 CB 气溶胶。在多个组织中测量了促炎生物标志物，包括动脉、大脑、肺和血浆。还在血浆中测定了心血管损伤的生物标志物。CB 吸入暴露增加了多个组织样本中的 CMI 相关促炎生物标志物，如 IFN-γ 和 TNFα。CB 暴露还诱导了血浆中的心血管损伤标志物（脂联素、MCP1、sE-选择素、sICam-1 和 TIMP1）。这些发现支持我们的动物暴露模型用于研究燃烧坑引起的 CMI 的有效性。未来的研究将模拟更多在多个燃烧坑现场记录的复杂有毒混合物。
