Weingand Nicole, Vuille-Dit-Bille Johanna, Jud Schefer Rahel, Kutter Annette P N, Stirn Martina, Adamik Katja-Nicole, Sigrist Nadja E
Department for Small Animals, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Department of Clinical Veterinary Science, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Animals (Basel). 2022 Aug 7;12(15):1996. doi: 10.3390/ani12151996.
Viscoelastic testing as a bedside test to assess global haemostasis has gained popularity in the past decade, with rotational thromboelastometry (ROTEM) and thromboelastography (TEG) being the two commonly used devices. TEG studies suggest analysis 30 min after blood sampling. However, the reproducibility of results over time for ROTEM analysis using lyophilized samples in dogs has not been established. In this study, we investigated the influence of time on viscoelastic testing, using 33 healthy staff-/client-owned dogs for blood sampling and repeated measurements of ROTEM tracings at three different time points after blood collection. Additionally, a group of 21 hospitalized patients with suspected coagulation disorders were included to investigate whether stability over time was comparable between healthy and ill dogs. We demonstrated a significant difference of ROTEM tracings over time, with a tendency towards hypocoagulability over time. These changes do have a clinical relevance as they exceed reference intervals and could therefore lead to erroneous conclusions about a patient's coagulation status. Therefore, time-specific reference intervals are proposed and presented in this publication.
在过去十年中，黏弹性测试作为一种用于评估整体止血功能的床旁检测方法越来越受欢迎，旋转血栓弹力图（ROTEM）和血栓弹力图（TEG）是两种常用的设备。TEG研究建议在采血后30分钟进行分析。然而，使用冻干样本对犬进行ROTEM分析时，结果随时间的可重复性尚未得到证实。在本研究中，我们使用33只健康的员工/客户拥有的犬进行采血，并在采血后的三个不同时间点重复测量ROTEM曲线，以研究时间对黏弹性测试的影响。此外，纳入了一组21例疑似凝血障碍的住院患者，以研究健康犬和患病犬随时间的稳定性是否具有可比性。我们证明了ROTEM曲线随时间存在显著差异，且随时间有低凝倾向。这些变化具有临床相关性，因为它们超出了参考区间，因此可能导致对患者凝血状态得出错误结论。因此，本出版物提出并给出了特定时间的参考区间。
