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SS-128的AI-2/Lux-S群体感应延长了虾的货架期(:从肌原纤维模拟到实际应用)

AI-2/Lux-S Quorum Sensing of SS-128 Prolongs the Shelf Life of Shrimp (: From Myofibril Simulation to Practical Application.

作者信息

Li Yuan, Liu Taige, Meng Xianghong, Qian Yilin, Yan Shijie, Liu Zunying

机构信息

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

College of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300392, China.

出版信息

Foods. 2022 Jul 29;11(15):2273. doi: 10.3390/foods11152273.

Abstract

Retarding the protein deterioration of shrimp during storage is important for maintaining its quality. SS-128 ( SS-128) is a biocontrol bacterium that can effectively maintain the fresh quality of food. This research establishes a myofibril simulation system and refrigerated control system to explore the impact of SS-128 on the quality and shelf life of refrigerated shrimp (). Through the bacterial growth assay and AI-2 signal molecule measurement, the effect of the AI-2/LuxS quorum sensing (QS) system of SS-128 and shrimp spoilage bacteria was established. In the myofibril simulation system, a study on protein degradation (dimer tyrosine content, protein solubility, sulfhydryl content, and carbonyl content) showed that adding SS-128 effectively slowed protein degradation by inhibiting the growth of food pathogens. The application to refrigerated shrimp indicated that the total volatile basic nitrogen (TVB-N) value increased more slowly in the group with added SS-128, representing better quality. The total viable count (TVC) and pH results exhibited similar trends. This study provides theoretical support for the application of SS-128 in storing aquatic products.

摘要

在储存过程中延缓虾的蛋白质变质对于保持其品质很重要。SS-128是一种能够有效维持食品新鲜品质的生物防治细菌。本研究建立了肌原纤维模拟系统和冷藏控制系统,以探究SS-128对冷藏虾品质和货架期的影响。通过细菌生长测定和AI-2信号分子测量,确定了SS-128的AI-2/LuxS群体感应(QS)系统对虾腐败菌的影响。在肌原纤维模拟系统中,一项关于蛋白质降解(二聚体酪氨酸含量、蛋白质溶解度、巯基含量和羰基含量)的研究表明,添加SS-128通过抑制食品病原体的生长有效地减缓了蛋白质降解。在冷藏虾上的应用表明,添加SS-128的组中总挥发性盐基氮(TVB-N)值增加得更慢,代表品质更好。总活菌数(TVC)和pH结果呈现相似趋势。本研究为SS-128在水产品储存中的应用提供了理论支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d74/9368648/37ae3d29caca/foods-11-02273-g001.jpg

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