Lee Woong-Jin, Cho Kyoungjoo, Kim Aaron-Youngjae, Kim Gyung-Whan
Department of Neurology, College of Medicine, Yonsei University, Seoul 03722, Korea.
Department of Life Science, Kyonggi University, Suwon 16227, Korea.
Materials (Basel). 2022 Jul 30;15(15):5269. doi: 10.3390/ma15155269.
Wound closure is a critical step in postoperative wound recovery. Substantial advancements have been made in many different means of facilitating wound closure, including the use of tissue adhesives. Compared to conventional methods, such as suturing, tissue bioadhesives better accelerate wound closure. However, several existing tissue adhesives suffer from cytotoxicity, inadequate tissue adhesive strength, and high costs. In this study, a series of bioadhesives was produced using non-swellable spider silk-derived silk fibroin protein and an outer layer of swellable polyethylene glycol and tannic acid. The gelation time of the spider silk-derived silk fibroin protein bioadhesive is less than three minutes and thus can be used during rapid surgical wound closure. By adding polyethylene glycol (PEG) 2000 and tannic acid as co-crosslinking agents to the N-Hydroxysuccinimide (NHS), and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) reaction, the adhesive strength of the bioadhesive became 2.5 times greater than that of conventional fibrin glue adhesives. Silk fibroin bioadhesives do not show significant cytotoxicity in vitro compared with other bioadhesives. In conclusion, silk fibroin bioadhesive is promising as a new medical tool for more effective and efficient surgical wound closure, particularly in bone fractures.
伤口闭合是术后伤口恢复的关键步骤。在促进伤口闭合的许多不同方法方面已经取得了重大进展，包括使用组织粘合剂。与传统方法（如缝合）相比，组织生物粘合剂能更好地加速伤口闭合。然而，现有的几种组织粘合剂存在细胞毒性、组织粘合强度不足和成本高等问题。在本研究中，使用不可膨胀的蜘蛛丝衍生丝素蛋白和可膨胀的聚乙二醇与单宁酸外层制备了一系列生物粘合剂。蜘蛛丝衍生丝素蛋白生物粘合剂的凝胶化时间少于三分钟，因此可用于快速手术伤口闭合。通过向N-羟基琥珀酰亚胺（NHS）和1-乙基-3-(3-二甲基氨基丙基)碳二亚胺（EDC）反应中添加聚乙二醇（PEG）2000和单宁酸作为共交联剂，生物粘合剂的粘合强度比传统纤维蛋白胶粘合剂提高了2.5倍。与其他生物粘合剂相比，丝素蛋白生物粘合剂在体外未表现出明显的细胞毒性。总之，丝素蛋白生物粘合剂有望成为一种新的医疗工具，用于更有效和高效的手术伤口闭合，特别是在骨折治疗中。
