Wang Ruofan, Zhu Jiangying, Jiang Guohua, Sun Yanfang, Ruan Liming, Li Pengfei, Cui Haiyan
Department of Polymer Materials, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
ACS Appl Bio Mater. 2020 Nov 16;3(11):7941-7951. doi: 10.1021/acsabm.0c01064. Epub 2020 Oct 21.
Wound dressing has been used for decades to be effective for accelerating skin wound healing. However, practical applications are still limited due to their lower cell affinity, tissue adhesiveness, and biocompatibility. Natural polymers are the important biomaterials because of their excellent biodegradability, biocompatibility, and low immunogenicity. In this work, the composite bioadhesives (PLS-CS/RSF) were prepared from regenerated silk fibroin (RSF) and polylysine-modified chitosan (PLS-CS) that were cross-linked by Ca ions. The adhesion property tests showed that the PLS-CS/RSF exhibited excellent bonding potentials for various substrates, and the adhesive strength was up to 70 kPa for isolated porcine skin by the extension test. The as-prepared PLS-CS/RSF was nontoxic, displayed obvious antibacterial effects against and , and their bacteriostasis rates were 100% after 120 min treatment. In addition, the PLS-CS/RSF exhibited favorable cytocompatibility by cell counting kit-8 assay. The animal model of wound closure results showed that PLS-CS/RSF can promote wound closure and the integrity of wound healing, inhibiting the secretion of inflammatory factor and tumor necrosis factor and stimulating vascular factor and α-smooth muscle actin to the release of vascular growth factor and promote angiogenesis during the process of wound healing by immunohistochemical assay.
伤口敷料已被使用数十年，以有效加速皮肤伤口愈合。然而，由于其较低的细胞亲和力、组织粘附性和生物相容性，其实际应用仍然有限。天然聚合物因其优异的生物降解性、生物相容性和低免疫原性而成为重要的生物材料。在这项工作中，复合生物粘合剂（PLS-CS/RSF）由再生丝素蛋白（RSF）和经聚赖氨酸修饰的壳聚糖（PLS-CS）制备而成，它们通过钙离子交联。粘附性能测试表明，PLS-CS/RSF对各种基材表现出优异的粘结潜力，通过拉伸试验，对离体猪皮的粘结强度高达70kPa。所制备的PLS-CS/RSF无毒，对[具体菌种1]和[具体菌种2]显示出明显的抗菌效果，处理120分钟后其抑菌率为100%。此外，通过细胞计数试剂盒-8检测，PLS-CS/RSF表现出良好的细胞相容性。伤口闭合的动物模型结果表明，PLS-CS/RSF可以促进伤口闭合和伤口愈合的完整性，通过免疫组织化学分析，在伤口愈合过程中抑制炎症因子和肿瘤坏死因子的分泌，并刺激血管因子和α-平滑肌肌动蛋白释放血管生长因子，促进血管生成。
