Laboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China.
Int J Mol Sci. 2022 Aug 5;23(15):8723. doi: 10.3390/ijms23158723.
Surface topography, protein adsorption, and the loading of coating materials can affect soft tissue sealing. Graphene oxide (GO) is a promising candidate for improving material surface functionalization to facilitate soft tissue integration between cells and biomaterials. In this study, TiO nanotubes (TNTs) were prepared by the anodization of Ti, and TNT-graphene oxide composites (TNT-GO) were prepared by subsequent electroplating. The aim of this study was to investigate the effect of TNTs and TNT-GO surface modifications on the behavior of human gingival fibroblasts (HGFs). Commercially pure Ti and TNTs were used as the control group, and the TNT-GO surface was used as the experimental group. Scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction were used to perform sample characterization. Cell adhesion, cell proliferation, cell immunofluorescence staining, a wound-healing assay, real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and Western blotting showed that the proliferation, adhesion, migration, and adhesion-related relative gene expression of HGFs on TNT-GO were significantly enhanced compared to the control groups, which may be mediated by the activation of integrin β1 and the MAPK-Erk1/2 pathway. Our findings suggest that the biological reactivity of HGFs can be enhanced by the TNT-GO surface, thereby improving the soft tissue sealing ability.
表面形貌、蛋白质吸附和涂层材料的负载都会影响软组织密封效果。氧化石墨烯（GO）是一种很有前途的候选材料，可用于改善材料表面功能化，促进细胞和生物材料之间的软组织整合。本研究通过 Ti 的阳极氧化制备了 TiO2 纳米管（TNTs），并通过随后的电镀制备了 TNT-氧化石墨烯复合材料（TNT-GO）。本研究旨在探讨 TNTs 和 TNT-GO 表面修饰对人牙龈成纤维细胞（HGFs）行为的影响。商用纯 Ti 和 TNTs 用作对照组，TNT-GO 表面用作实验组。采用扫描电子显微镜、X 射线光电子能谱和 X 射线衍射对样品进行了表征。细胞黏附、细胞增殖、细胞免疫荧光染色、划痕愈合试验、实时逆转录聚合酶链反应（RT-PCR）和 Western blot 显示，与对照组相比，HGFs 在 TNT-GO 上的增殖、黏附、迁移和黏附相关基因的相对表达显著增强，这可能是通过整合素β1 和 MAPK-Erk1/2 通路的激活介导的。我们的研究结果表明，TNT-GO 表面可以增强 HGFs 的生物反应性，从而提高软组织密封能力。
