Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy.
Department of Physics and Astronomy, University of Catania, 95123 Catania, Italy.
Int J Mol Sci. 2022 Aug 8;23(15):8820. doi: 10.3390/ijms23158820.
The cultivation of soilless tomato in greenhouses has increased considerably, but little is known about the assembly of the root microbiome compared to plants grown in soil. To obtain such information, we constructed an assay in which we traced the bacterial and fungal communities by amplicon-based metagenomics during the cultivation chain from nursery to greenhouse. In the greenhouse, the plants were transplanted either into agricultural soil or into coconut fiber bags (soilless). At the phylum level, bacterial and fungal communities were primarily constituted in all microhabitats by Proteobacteria and Ascomycota, respectively. The results showed that the tomato rhizosphere microbiome was shaped by the substrate or soil in which the plants were grown. The microbiome was different particularly in terms of the bacterial communities. In agriculture, enrichment has been observed in putative biological control bacteria of the genera and and in potential phytopathogenic fungi. Overall, the study describes the different shaping of microbial communities in the two cultivation methods.
温室无土栽培番茄的种植量有了显著增加，但与土壤种植相比，人们对其根系微生物组的组装情况知之甚少。为了获取相关信息，我们构建了一个分析方法，通过基于扩增子的宏基因组学，在从苗圃到温室的整个种植链中对细菌和真菌群落进行追踪。在温室中，植物要么被移植到农业土壤中，要么被移植到椰子纤维袋（无土）中。在门水平上，在所有微生境中，细菌和真菌群落分别主要由变形菌门和子囊菌门构成。结果表明，番茄根际微生物组由植物生长的基质或土壤所塑造。特别是在细菌群落方面，微生物组存在明显差异。在农业种植中，观察到了属 和 中具有潜在生物防治作用的细菌以及潜在的植物病原菌真菌的富集。总的来说，该研究描述了两种种植方法中微生物群落的不同塑造方式。
