Sudmoon Runglawan, Kaewdaungdee Sanit, Ameamsri Unchaleeporn, Tanee Tawatchai, Siripiyasing Pornnarong, Wonok Warin, Chaveerach Arunrat
Faculty of Law, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Plants (Basel). 2022 Jul 30;11(15):1985. doi: 10.3390/plants11151985.
α-EG is a unique substance that was first found in the leaves and fruits of (Mc) growing in Thailand using GC-MS at 52.33% and 54.12%. It was then concentrated and its abundance quantified, along with that of pinoresinol, via GC, compared to the standards in leaves, ufp, rfp, rawfs, and seeds. α-EG and pinoresinol, which have collagen stimulating, skin whitening, and an inhibitory effect on wrinkle formation, were found in different concentrations and amounts. Three different concentrations of the five Mc part extracts were tested on for gene expression related to the aforementioned activities, , , and , and by qRT-PCR. The results showed various expression levels, both stimulatory and inhibitory, with different concentrations of plant parts and genes. Similar results were revealed when the experiments were performed with (Ma), which was found to contain 20.48 g protein p/100 g leaves at concentrations of 3.11 mg/mL. The studied Mc parts seem to have advantages based on the stated objectives, gene type and level of activity of each plant part. Rawfs and leaves supplemented with Ma samples were selected for toxicity tests with PBMCs. The lack of both cell and DNA toxicity from the rawfs indicated that they can be used safely.
α - 乙氧基没食子酸是一种独特的物质，最初是在泰国生长的（某种植物，文中未完整写出植物名称，用“(Mc)”表示）的叶子和果实中通过气相色谱 - 质谱联用仪（GC - MS）发现的，含量分别为52.33%和54.12%。然后将其浓缩，并通过气相色谱（GC）对其丰度以及松脂醇的丰度进行定量，与叶子、未成熟果实、成熟果实、粗提物和种子中的标准品进行比较。发现具有刺激胶原蛋白生成、美白皮肤以及抑制皱纹形成作用的α - 乙氧基没食子酸和松脂醇，其浓度和含量各不相同。对（某种植物，文中未完整写出植物名称，用“(Mc)”表示）的五个部分提取物的三种不同浓度进行了测试，通过实时荧光定量聚合酶链反应（qRT - PCR）检测与上述活性相关的基因表达，即 、 、 、 和 。结果表明，不同浓度的植物部分和基因呈现出刺激和抑制等不同的表达水平。当用（某种植物，文中未完整写出植物名称，用“(Ma)”表示）进行实验时也得到了类似结果，发现其叶子中每100克含有20.48克蛋白质，浓度为3.11毫克/毫升。基于所述目标、基因类型以及每个植物部分的活性水平，所研究的（Mc）植物部分似乎具有优势。选择粗提物和添加了（Ma）样品的叶子进行外周血单核细胞（PBMCs）毒性测试。粗提物对细胞和DNA均无毒性，表明其可安全使用。
