Benhamou S, Callais F, Sancho-Garnier H, Min S, Courtois Y A, Festy B
Eur J Cancer Clin Oncol. 1986 Dec;22(12):1489-93. doi: 10.1016/0277-5379(86)90085-4.
A cohort study of 29 nurses who extensively handle cytostatic drugs, 29 controls matched on sex and age, and seven patients under chemotherapy was carried out between 1983 and 1985. In a first study, urinary mutagenicity assays performed with the Ames test towards Salmonella typhimurium TA 98 with and without S9 mix gave an increased mutagenic activity, although not significant, for nurses as compared to controls, after adjustment on smoking habits. The results of mutagenicity assays for patients were significantly higher (P less than 0.01) than for non-smoker nurses and non-smoker controls with TA 98 without S9 mix. Of the 29 pairs, complementary assays were performed for non-smokers, that is 11 nurses and 11 matched controls, with TA 98, TA 100 and TA 1535 +/- S9 mix. A significant increase in mutagenic activity (P less than 0.05) was detected for nurses as compared to matched controls towards TA 98 with and without S9 mix. Moreover, the mutagenicity was significantly increased (P less than 0.05) in nurses who handled at least one electrophilic agents as compared to nurses who handled non-electrophilic drugs towards TA 98 with and without S9 mix.
1983年至1985年间，对29名大量接触细胞毒性药物的护士、29名年龄和性别相匹配的对照者以及7名接受化疗的患者进行了一项队列研究。在第一项研究中，采用艾姆斯试验对鼠伤寒沙门氏菌TA 98进行尿致突变性检测，无论有无S9混合物，在根据吸烟习惯进行调整后，与对照组相比，护士的致突变活性有所增加，尽管不显著。在无S9混合物的情况下，对TA 98进行检测时，患者的致突变性检测结果显著高于不吸烟的护士和不吸烟的对照者（P<0.01）。在29对研究对象中，对不吸烟者（即11名护士和11名匹配的对照者）采用TA 98、TA 100和TA 1535±S9混合物进行了补充检测。与匹配的对照者相比，无论有无S9混合物，护士对TA 98的致突变活性均显著增加（P<0.05）。此外，与处理非亲电药物的护士相比，处理至少一种亲电药物的护士在有无S9混合物的情况下对TA 98的致突变性均显著增加（P<0.05）。
