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原花青素结构与其在帕金森病模型中保护作用的关系。

The Relationship between Procyanidin Structure and Their Protective Effect in a Parkinson's Disease Model.

机构信息

Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310000, China.

出版信息

Molecules. 2022 Aug 6;27(15):5007. doi: 10.3390/molecules27155007.

DOI:10.3390/molecules27155007
PMID:35956957
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9370466/
Abstract

This study evaluated the effect of grape seed-derived monomer, dimeric, and trimeric procyanidins on rat pheochromocytoma cell line (PC12) cells and in a zebrafish Parkinson's disease (PD) model. PC12 cells were cultured with grape seed-derived procyanidins or deprenyl for 24 h and then exposed to 1.5 mm 1-methyl-4-phenylpyridinium (MPP) for 24 h. Zebrafish larvae (AB strain) 3 days post-fertilization were incubated with deprenyl or grape seed-derived procyanidins in 400 µM 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) for 4 days. The results showed that the procyanidin dimers procyanidin B1 (B1), procyanidin B2 (B2), procyanidin B3 (B3), procyanidin B4 (B4), procyanidin B1-3--gallate (B1-G), procyanidin B2-3--gallate (B2-G), and the procyanidin trimer procyanidin C1 (C1) had a protective effect on PC12 cells, decreasing the damaged dopaminergic neurons and motor impairment in zebrafish. In PC12 cells and the zebrafish PD model, procyanidin (B1, B2, B3, B4, B1-G, B2-G, C1) treatment decreased the content of reactive oxygen species (ROS) and malondialdehyde (MDA), increased the activity of antioxidant enzymes glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD), and upregulated the expression of nuclear factor-erythroid 2-related factor (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), and heme oxygenase-1 (HO-1). These results suggest that in PC12 cells and the zebrafish PD model, the neuroprotective effects of the procyanidins were positively correlated with their degree of polymerization.

摘要

本研究评估了葡萄籽来源的单体、二聚体和三聚体原花青素对大鼠嗜铬细胞瘤细胞系（PC12）细胞和斑马鱼帕金森病（PD）模型的影响。将 PC12 细胞与葡萄籽来源的原花青素或丙炔醇一起培养 24 小时，然后用 1.5mm 1-甲基-4-苯基吡啶鎓（MPP）处理 24 小时。受精后 3 天的斑马鱼幼虫（AB 品系）在 400µM 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）中与丙炔醇或葡萄籽来源的原花青素一起孵育 4 天。结果表明，原花青素二聚体原花青素 B1（B1）、原花青素 B2（B2）、原花青素 B3（B3）、原花青素 B4（B4）、原花青素 B1-3-没食子酸酯（B1-G）、原花青素 B2-3-没食子酸酯（B2-G）和原花青素三聚体原花青素 C1（C1）对 PC12 细胞具有保护作用，减少多巴胺能神经元损伤和斑马鱼的运动障碍。在 PC12 细胞和斑马鱼 PD 模型中，原花青素（B1、B2、B3、B4、B1-G、B2-G、C1）处理降低了活性氧（ROS）和丙二醛（MDA）的含量，增加了谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性，并上调了核因子-红细胞 2 相关因子（Nrf2）、NAD（P）H：醌氧化还原酶 1（NQO1）和血红素加氧酶-1（HO-1）的表达。这些结果表明，在 PC12 细胞和斑马鱼 PD 模型中，原花青素的神经保护作用与其聚合度呈正相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c8d/9370466/b839b69993c4/molecules-27-05007-g011.jpg
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