Dong Chuanjiang, Chen Wei, Zou Lili, Liu Binbin, Deng Kaihong, Guo Dingrui, Wang Peng, Chen Hao, Wang Helen, Wang Jun
The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Medical College, China Three Gorges University, Yichang, China.
Front Microbiol. 2022 Jul 25;13:963901. doi: 10.3389/fmicb.2022.963901. eCollection 2022.
is a foodborne zoonotic bacterium that is pathogenic to guinea pigs, rabbits, and mice. It also causes pseudotuberculosis in humans. However, it still lacked the scientific basis for control. Here, we found out that Ebselen (EbSe) exhibited synergistic antibacterial activity with silver nitrate (Ag) against YpIII strain with high efficacy using UV-visible light absorption spectrum, 5,5'-dithiobis-(2-nitrobenzoic acid), laser scanning confocal microscope, flow cytometry, transmission electron microscopy and Western blotting assays. The depletion of total glutathione (GSH) amount and inhibition of thioredoxin reductase (TrxR) activity in thiol-dependent redox system revealed the destructiveness of EbSe-Ag-caused intracellular oxidative stress. Furthermore, a YpIII-caused mice gastroenteritis model was constructed. EbSe-Ag significantly reduced bacterial loads with low toxicity. It also down-regulated the expression levels of interferon (IL)-1β and tumor necrosis factor-α, up-regulated the expression level of IL-10 on-site. All the results demonstrated the antibacterial activity and immune-modulatory property of EbSe-Ag. Collectively, these results provided academic fundament for further analysis and development of EbSe-Ag as the antibacterial agents for pseudotuberculosis control.
是一种食源性人畜共患病细菌，对豚鼠、兔子和小鼠具有致病性。它还会导致人类患假结核。然而，它仍然缺乏控制的科学依据。在此，我们通过紫外可见吸收光谱、5,5'-二硫代双-(2-硝基苯甲酸)、激光扫描共聚焦显微镜、流式细胞术、透射电子显微镜和蛋白质免疫印迹分析发现，依布硒仑（EbSe）与硝酸银（Ag）对YpIII菌株表现出协同抗菌活性，且效果显著。硫醇依赖性氧化还原系统中总谷胱甘肽（GSH）量的消耗和硫氧还蛋白还原酶（TrxR）活性的抑制揭示了EbSe-Ag引起的细胞内氧化应激的破坏性。此外，构建了YpIII引起的小鼠肠胃炎模型。EbSe-Ag能显著降低细菌载量且毒性低。它还下调了干扰素（IL）-1β和肿瘤坏死因子-α的表达水平，上调了IL-10在局部的表达水平。所有结果都证明了EbSe-Ag的抗菌活性和免疫调节特性。总的来说，这些结果为进一步分析和开发EbSe-Ag作为控制假结核的抗菌剂提供了理论基础。
