衣原体抗体的单抗原免疫荧光检测
Single-antigen immunofluorescence test for chlamydial antibodies.
作者信息
Saikku P, Paavonen J
出版信息
J Clin Microbiol. 1978 Aug;8(2):119-22. doi: 10.1128/jcm.8.2.119-122.1978.
A simple method is presented for producing large numbers of inclusions of Chlamydia trachomatis serotype L2 in cell cultures on slides for immunofluorescence antibody staining. Preliminary results with a total of 1,241 human sera from different groups were consistent with findings by earlier methods: 82% of chlamydia-positive men with nongonococcal urethritis had antibodies at titers of greater than or equal to 8, with a geometric mean titer (GMT) of 44.9; 68.5% of chlamydia-negative men with nongonococcal urethritis had titers of greater than or equal to 8, with a GMT of 38.6; 27% of male blood donors had titers of greater than or equal to 8, with a GMT of 19.6; 95.0% of chlamydia-positive women had titers of greater than or equal to 8, with a GMT of 80.3; 67.0% of chlamydia-negative partners of men with nongonococcal urethritis had titers of greater than or equal to 8, with a GMT of 47.0; 50.2% of control women had titers of greater than or equal to 8, with a GMT of 27.7% and 8.1% of children (aged 1 to 15 years) had titers of greater than or equal to 8, with a GMT of 17.8.
本文介绍了一种简单的方法，可在载玻片上的细胞培养物中大量产生沙眼衣原体L2血清型包涵体，用于免疫荧光抗体染色。对来自不同组别的总共1241份人血清的初步结果与早期方法的结果一致：82%的衣原体阳性非淋菌性尿道炎男性抗体滴度大于或等于8，几何平均滴度（GMT）为44.9；68.5%的衣原体阴性非淋菌性尿道炎男性滴度大于或等于8，GMT为38.6；27%的男性献血者滴度大于或等于8，GMT为19.6；95.0%的衣原体阳性女性滴度大于或等于8，GMT为80.3；67.0%的非淋菌性尿道炎男性的衣原体阴性伴侣滴度大于或等于8，GMT为47.0；50.2%的对照女性滴度大于或等于8，GMT为27.7%，8.1%的儿童（1至15岁）滴度大于或等于8，GMT为17.8。
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