Bell J D, Sadler P J, Macleod A F, Turner P R, La Ville A
FEBS Lett. 1987 Jul 13;219(1):239-43. doi: 10.1016/0014-5793(87)81224-3.
Single-pulse and Hahn spin-echo 500 MHz 1H NMR spectra of human blood plasma and isolated chylomicrons, VLDL, LDL and HDL are reported. The comparison has enabled specific assignments to be made for the resonances of individual lipoproteins in the CH2 and CH3 (fatty acid), and NMe+3 (phospholipid choline head group) regions of the spectra of plasma (0.8-1.3 and approximately 3.25 ppm, respectively). Fasting, and freeze-thawing of plasma samples led to marked changes in the intensities and linewidths of lipid resonances. Analysis of lipid resonances in the spectra of plasma in terms of individual lipoproteins may shed new light on many conditions of clinical and biochemical interest.
报道了人体血浆以及分离出的乳糜微粒、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的单脉冲和哈恩自旋回波500兆赫1H核磁共振谱。通过比较，能够对血浆谱中CH2和CH3（脂肪酸）以及NMe+3（磷脂胆碱头部基团）区域（分别为0.8 - 1.3 ppm和约3.25 ppm）中各个脂蛋白的共振进行具体归属。血浆样本的禁食和冻融导致脂质共振的强度和线宽发生显著变化。根据各个脂蛋白对血浆谱中的脂质共振进行分析，可能会为许多具有临床和生化意义的情况提供新的线索。
