Bell J D, Brown J C, Norman R E, Sadler P J, Newell D R
Department of Chemistry, Birkbeck College, University of London, Christopher Ingold Laboratories, UK.
NMR Biomed. 1988 Apr;1(2):90-4. doi: 10.1002/nbm.1940010206.
Single pulse and Hahn spin-echo 400 and 500 MHz 1H NMR spectra from normal subjects and cancer patients are reported. No significant difference was found between the average -CH3 and -(CH2)n- lipoprotein linewidths of the two groups in contrast to a previous report. Such measurements are shown to be complicated by freezing and thawing of samples which broadens lipoprotein resonances, and by eating which sharpens them. Deconvolution of the composite lipoprotein signals enables these effects to be interpreted. Other notable features of plasma spectra are discussed including resonances for 'acute-phase' glycoproteins, ketone bodies and other small molecules.
报告了来自正常受试者和癌症患者的单脉冲和哈恩自旋回波400兆赫和500兆赫的1H核磁共振谱。与之前的一份报告相反，两组的平均-CH3和-(CH2)n-脂蛋白线宽之间未发现显著差异。结果表明，样品的冷冻和解冻会使脂蛋白共振变宽，而进食会使共振变尖锐，这使得此类测量变得复杂。复合脂蛋白信号的反褶积能够解释这些影响。文中还讨论了血浆光谱的其他显著特征，包括“急性期”糖蛋白、酮体和其他小分子的共振。
