Wang Xiaoyan, Pu Xiaoyu, Chen Lijia, Guo Miaoying, Zheng Changbo, Wang Hongtian, Wang Xueyan
Department of Allergy，Beijing Shijitan Hospital，Capital Medical University，Beijing，100038，China.
Allergy Center，Beijing Shijitan Hospital，Capital Medical University.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2022 Aug;36(8):576-581. doi: 10.13201/j.issn.2096-7993.2022.08.002.
To study the differences and clinical significance of dust mite allergen components in allergic rhinitis（AR） and allergic rhinitis with asthma syndrome（ARAS） patients. The clinical data of 42 AR patients were retrospectively analyzed and patients were divided into AR and ARAS group. The serum sIgE concentrations of house dust mites were detected by ImmunoCAP system. The allergen components of Der p（Der p 1, Der p 2, Der p 7, Der p 10, Der p 21, Der p 23） and Der f（Der f 1, Der f 2） were analyzed by protein microarray method. The concentration differences of dust mite allergen and its components in AR and ARAS groups were analyzed. Thirty-one cases of AR and 11 cases of ARAS were included. The positive rate of Der p and Der f was 100.0% and 97.6%, respectively. The highest sensitization rates of Der p allergen components were as following: Der p 1（73.8%）, Der f 1（66.7%）, Der f 2（64.3%） and Der p 2（61.9%）. The sensitization rates of Der f 1（100.0% vs 54.8%, =0.006）, Der p 2（90.9% vs 51.6%, =0.021） and Der f 2（100.0% vs 51.6%, =0.004） in ARAS group were significantly higher than those in AR group. The sIgE concentrations of Der p in AR group were significantly lower than those in ARAS group（[7.65±12.15]kUA/L vs[15.20±18.77]kUA/L, <0.05）. The sIgE concentrations of Der p 1（[5.39±4.61]kUA/L vs[2.03±2.97]kUA/L, =0.013）, Der p 2（[8.82± 13.58]kUA/L vs[2.78±5.80]kUA/L, =0.001）, Der p 23（[1.76± 3.88]kUA/L vs[0.28±0.65]kUA/L, <0.001） was significantly higher in ARAS group than that of AR group. Correlation analysis showed that Der p 1, Der p 2, Der f 1 and Der f 2 had high positive correlation（<0.01）. The dust mite components sensitization showed a multiple-sensitized mode. 66.7% of the 42 patients were positive for two or more components while it was 58.1% of the AR group and 90.9% of the ARAS group. The sensitization rate of 3 or more components in ARAS group was significantly higher than that in AR group（54.6% vs 29.1%, <0.05）. The concentration of dust mites allergens in ARAS group is higher than that in AR group. Der p 1, Der f 1, Der p 2 and Der f 2 are the main allergen components with a higher sensitization rate in ARAS group. The concentrations of Der p 1, Der p 2 and Der p 23 were higher in ARAS group. The ARAS group is prone to multi-sensitzed to allergen components.
研究变应性鼻炎（AR）和变应性鼻炎合并哮喘综合征（ARAS）患者尘螨变应原成分的差异及临床意义。回顾性分析42例AR患者的临床资料，将患者分为AR组和ARAS组。采用免疫捕获系统检测屋尘螨血清特异性免疫球蛋白E（sIgE）浓度。采用蛋白质芯片法分析粉尘螨Der p（Der p 1、Der p 2、Der p 7、Der p 10、Der p 21、Der p 23）和Der f（Der f 1、Der f 2）的变应原成分。分析AR组和ARAS组尘螨变应原及其成分的浓度差异。纳入31例AR患者和11例ARAS患者。Der p和Der f的阳性率分别为100.0%和97.6%。Der p变应原成分的最高致敏率依次为：Der p 1（73.8%）、Der f 1（66.7%）、Der f 2（64.3%）和Der p 2（61.9%）。ARAS组Der f 1（100.0%比54.8%，P = 0.006）、Der p 2（90.9%比51.6%，P = 0.021）和Der f 2（100.0%比51.6%，P = 0.004）的致敏率显著高于AR组。AR组Der p的sIgE浓度显著低于ARAS组（[7.65±12.15]kUA/L比[15.20±18.77]kUA/L，P<0.05）。ARAS组Der p 1（[5.39±4.61]kUA/L比[2.03±2.97]kUA/L，P = 0.013）、Der p 2（[8.82±13.58]kUA/L比[2.78±5.80]kUA/L，P = 0.001）、Der p 23（[1.76±3.88]kUA/L比[0.28±0.65]kUA/L，P<0.001）的sIgE浓度显著高于AR组。相关性分析显示，Der p 1、Der p 2、Der f 1和Der f 2呈高度正相关（P<0.01）。尘螨成分致敏呈多致敏模式。42例患者中66.7%对两种或两种以上成分呈阳性，而AR组为58.1%，ARAS组为90.9%。ARAS组3种或3种以上成分的致敏率显著高于AR组（54.6%比29.1%，P<0.05）。ARAS组尘螨变应原浓度高于AR组。Der p 1、Der f 1、Der p 2和Der f 2是主要变应原成分，在ARAS组致敏率较高。ARAS组Der p 1、Der p 2和Der p 23的浓度较高。ARAS组易对变应原成分产生多致敏。
