sequentially extracted leaf 中文可译为“依次提取的叶片”。 因此，该译文为： 依次提取的叶耳草（Rejicho）的 Vernonia auriculifera Hiern 的抗菌评估。
Antimicrobial Evaluation of Sequentially extracted Leaf of Vernonia auriculifera Hiern (Rejicho).
机构信息
Pharmacy Department, Arba Minch University, Arba Minch, Ethiopia.
School of Pharmacy, Jimma University, Jimma, Ethiopia.
出版信息
BMC Complement Med Ther. 2022 Aug 12;22(1):219. doi: 10.1186/s12906-022-03690-2.
BACKGROUND
Though there are reports about V. auriculifera antimicrobial activity, there is not enough information about its activity on some bacterial and fungal species. Besides, there was no quantitative evaluation done for phytochemicals previously. Therefore, the main purpose of this research work is to evaluate the antimicrobial activity and quantitative evaluation of the phenol and flavonoid contents of V. auriculifera.
OBJECTIVES
The objective of this study was to conduct the antimicrobial and quantitative evaluation of a sequentially extracted leaf of Vernonia auriculifera Hiern.
METHODS
The leaves of the plant were cleaned with tap water and air-dried. The grounded leaf product was subsequently extracted by hexane, chloroform, and methanol in maceration flasks. Then the total phenol and total flavonoid content in each extract were determined. Standard strains of bacterial and fungal species were used to assess the antibacterial, and antifungal susceptibility test and to determine the minimum inhibitory concentration of crude extract.
RESULT
Extraction yield for hexane, chloroform, and methanol was 0.6 ± 0.05%, 1.7 ± 0.02%, and 3.3 ± 0.01% respectively. The total phenolic content of methanol extract was 72.998 ± 0.002 mg GAE /g. The total flavonoid content of hexane and chloroform extracts were 2.59 ± 0.004 mg QE/g and 9.6 ± 0.02 mg QE/g respectively. The antimicrobial activity test showed the chloroform extract was the most active against all test microorganisms.
CONCLUSION
This study has shown the activity of V. auriculifera against selected microorganisms of study. The chloroform extract was the most active as compared to the hexane and methanol extracts.
背景
尽管有关于 V. auriculifera 抗菌活性的报道，但关于其对某些细菌和真菌物种的活性的信息还不够充分。此外，以前没有对植物化学物质进行定量评估。因此，这项研究工作的主要目的是评估 V. auriculifera 的抗菌活性和酚类及类黄酮含量的定量评估。
目的
本研究旨在对 Vernonia auriculifera Hiern 的叶进行抗菌活性和定量评估。
方法
用自来水清洗植物的叶子，然后风干。将研磨后的叶产物在索氏提取器中用己烷、氯仿和甲醇提取。然后测定各提取物中的总酚和总黄酮含量。使用标准的细菌和真菌菌株评估抗菌和抗真菌药敏试验，并确定粗提物的最小抑菌浓度。
结果
己烷、氯仿和甲醇的提取产率分别为 0.6±0.05%、1.7±0.02%和 3.3±0.01%。甲醇提取物的总酚含量为 72.998±0.002 mg GAE/g。己烷和氯仿提取物的总黄酮含量分别为 2.59±0.004 mg QE/g 和 9.6±0.02 mg QE/g。抗菌活性试验表明，氯仿提取物对所有测试的微生物均具有最强的活性。
结论
本研究表明 V. auriculifera 对所选研究微生物具有活性。与己烷和甲醇提取物相比，氯仿提取物的活性最强。
Zotero 插件