Ashenafi Ephrem, Abula Teferra, Abay Solomon Mequanente, Arayaselassie Mahlet, Taye Samson, Muluye Rekik Ashebir
Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Department of Pathology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
J Exp Pharmacol. 2023 Jan 27;15:29-40. doi: 10.2147/JEP.S398487. eCollection 2023.
The leaves of has been used traditionally for the treatment of inflammatory disorders, and pain in various parts of Ethiopia. However, to our knowledge, the analgesic and anti-inflammatory activity of the crude extract and solvent fractions has never been experimentally studied.
To assess the analgesic and anti-inflammatory activities of leaf extract and solvent fractions in rodent models.
Air-dried leaves of were grounded and macerated using 80% methanol. The air-dried, grounded leaves were also successively extracted with ethyl acetate, and methanol. The residue was then macerated in water for 72 hr. The extract's peripheral analgesic activity, as well as the solvent fractions, were determined using an acetic acid-induced writhing test. The hot plate model was used to assess the central analgesic effect. Carrageenan-induced hind paw edema and cotton pellet-induced granuloma models were used to assess the anti-inflammatory effect in rats.
The 80% methanol leaf extract and solvent fractions have demonstrated significant (p < 0.05) peripheral and central analgesic activity. Both 80% methanol leaf extract and solvent fractions of were found to have anti-inflammatory activity in a carrageenan-induced rat paw edema model. In the cotton pellet-induced granuloma model, all concentrations of 80% methanol leaf extract (ME), methanol fraction (MEF), and aqueous fractions (AQF) of inhibited exudate and granuloma formation. Although all tested doses significantly inhibited granuloma mass formation, only the medium and highest ethyl acetate fraction (EAF) doses significantly inhibited the generation of inflammatory exudate.
This study's findings indicate that the solvent fractions and 80% methanol extract of have analgesic and anti-inflammatory properties. This study's findings not only confirm the plants' traditional claim but also provide clues for further investigation of the active principles of this plant for the development of effective and safe analgesic and anti-inflammatory drugs.
在埃塞俄比亚的不同地区，[植物名称]的叶子传统上被用于治疗炎症性疾病和疼痛。然而，据我们所知，该植物粗提物和溶剂萃取部位的镇痛和抗炎活性从未经过实验研究。
评估[植物名称]叶提取物及其溶剂萃取部位在啮齿动物模型中的镇痛和抗炎活性。
将[植物名称]的风干叶研磨并使用80%甲醇浸软。风干并研磨后的叶子还依次用乙酸乙酯和甲醇进行萃取。然后将残渣在水中浸软72小时。使用醋酸诱导扭体试验测定提取物及其溶剂萃取部位的外周镇痛活性。采用热板模型评估中枢镇痛作用。使用角叉菜胶诱导的后爪水肿模型和棉球诱导的肉芽肿模型评估大鼠的抗炎作用。
80%甲醇叶提取物及其溶剂萃取部位均表现出显著（p<0.05）的外周和中枢镇痛活性。在角叉菜胶诱导的大鼠爪水肿模型中，发现80%甲醇叶提取物及其溶剂萃取部位均具有抗炎活性。在棉球诱导的肉芽肿模型中，[植物名称]的所有浓度的80%甲醇叶提取物（ME）、甲醇萃取部位（MEF）和水萃取部位（AQF）均抑制渗出物和肉芽肿形成。尽管所有测试剂量均显著抑制肉芽肿块形成，但只有中等剂量和高剂量的乙酸乙酯萃取部位（EAF）显著抑制炎性渗出物的产生。
本研究结果表明，[植物名称]的溶剂萃取部位和80%甲醇提取物具有镇痛和抗炎特性。本研究结果不仅证实了该植物的传统药用价值，还为进一步研究该植物的活性成分以开发有效且安全的镇痛和抗炎药物提供了线索。
