Key Laboratory of Healthy Food Development and Nutrition Regulation of China National Light Industry, School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China; School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
Int J Biol Macromol. 2022 Oct 31;219:876-885. doi: 10.1016/j.ijbiomac.2022.08.043. Epub 2022 Aug 10.
In this study, three polysaccharides (BGPs: BGPs-Z21, BGPs-Z23, and BGPs-Z31) were successively extracted from barley (Hordeum vulgare L.) grasses (BG) at different growth stages, including seedling (Z21), tillering (Z23), and stem elongation (Z31). The effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical characteristics and biological activities of BGPs were investigated and compared. Results showed that the simulated saliva-gastrointestinal digestion had considerable influences on reducing sugar content, chemical components, monosaccharide constituents, and molecular weights of BGPs but hardly affected their preliminarily structural characteristics. Moreover, the antioxidant activities of BGPs were weakened after the simulated saliva-gastrointestinal digestion, but their bile acid-binding capacities were remarkably enhanced. The digested BGPs-Z31 by gastric juice possessed better antioxidant benefit, and bile acid-binding capacity (80.33 %) than other digested products. Overall, these results indicated that BGPs obtained from BG are valuable for functional foods as promising bioactive ingredients.
在这项研究中，从大麦（Hordeum vulgare L.）草（BG）的不同生长阶段（幼苗[Z21]、分蘖[Z23]和茎伸长[Z31]）中成功地提取了三种多糖（BGPs：BGPs-Z21、BGPs-Z23 和 BGPs-Z31）。研究并比较了体外模拟唾液-胃肠消化对 BGPs 的理化特性和生物活性的影响。结果表明，模拟唾液-胃肠消化对还原糖含量、化学成分、单糖组成和 BGPs 的分子量有很大的影响，但对其初步结构特性影响不大。此外，BGPs 的抗氧化活性在模拟唾液-胃肠消化后减弱，但它们的胆汁酸结合能力显著增强。胃液消化的 BGPs-Z31 具有更好的抗氧化功效和胆汁酸结合能力（80.33%），优于其他消化产物。总的来说，这些结果表明，BG 中提取的 BGPs 作为有前途的生物活性成分，是功能性食品的宝贵选择。
