Jiang Shan, Zhang Sha, Jiang Xiangdong, Tian Shuge
College of Traditional Chinese Medicine, Xinjiang Medical University, Shangde North Road, Shuimogou District, Urumqi, 830054, China.
The Fifth Clinical Medical College, Xinjiang Medical University, Shangde North Road, Shuimogou District, Urumqi, 830054, China.
Bioresour Bioprocess. 2023 Jan 21;10(1):5. doi: 10.1186/s40643-023-00624-9.
In order to meet the contemporary concept of sustainable development, the reuse of biological waste has also been emphasized. Lots of papers nowadays study the extraction of primary residues. The disposal of secondary residues is often neglected. The chemical composition and biological activity of secondary residues of Turkish Gall (SRTG) were researched in this paper. We selected five methods to extract the SRTG, and the extraction conditions were water, hydrochloric acid buffer (pH = 2), artificial gastric juice (pH = 2), phosphate buffer (pH = 6.8), and artificial intestinal solution (pH = 6.8). The changes of phenolic components were determined by spectrophotometry and high-performance liquid chromatography. The acid-base environment did not affect total polyphenols contents and gallic acid ethyl ester contents in SRTG. But it affected the gallic acid contents in SRTG. The contents of gallic acid in the hydrochloric acid buffer extraction groups were 1.63 times that of the water extraction group. The SRTG were extracted by hydrochloric acid buffer also had better inhibition on Escherichia coli and Staphylococcus aureus. In addition, SRTG showed positive effects on 1,1-Diphenyl-2-picrylhydrazyl Free, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ·OH radicals, and Ferric ion reducing antioxidant power. Some active components of SRTG can be effectively released through the digestion of simulated gastric juices in vitro. The change of active ingredients affects the antibacterial and antioxidant capacity. The results provide data support for the conversion of secondary residues into products, such as feed additives. The SRTG has certain contributes to the value of the circular economy.
为了符合当代可持续发展理念，生物废弃物的再利用也得到了重视。如今许多论文研究初级残渣的提取。二级残渣的处理往往被忽视。本文研究了五倍子二级残渣（SRTG）的化学成分和生物活性。我们选择了五种方法提取SRTG，提取条件分别为水、盐酸缓冲液（pH = 2）、人工胃液（pH = 2）、磷酸盐缓冲液（pH = 6.8）和人工肠液（pH = 6.8）。通过分光光度法和高效液相色谱法测定酚类成分的变化。酸碱环境不影响SRTG中总多酚含量和没食子酸乙酯含量，但影响SRTG中没食子酸含量。盐酸缓冲液提取组中没食子酸含量是水提取组的1.63倍。用盐酸缓冲液提取的SRTG对大肠杆菌和金黄色葡萄球菌也有较好的抑制作用。此外，SRTG对1,1-二苯基-2-苦基肼自由基、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）、·OH自由基和铁离子还原抗氧化能力均表现出积极作用。SRTG的一些活性成分可通过体外模拟胃液消化有效释放。活性成分的变化影响抗菌和抗氧化能力。研究结果为二级残渣转化为饲料添加剂等产品提供了数据支持。SRTG对循环经济价值有一定贡献。
