NanoGlycobiology Unit, Department of NanoBiotechnology, Universität für Bodenkultur Wien, Vienna, Austria.
Clinic of Conservative and Preventive Dentistry, Division of Clinical Oral Microbiology and Immunology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland.
Mol Oral Microbiol. 2023 Apr;38(2):115-133. doi: 10.1111/omi.12385. Epub 2022 Aug 29.
The recently identified bacterium Tannerella serpentiformis is the closest phylogenetic relative of Tannerella forsythia, whose presence in oral biofilms is associated with periodontitis. Conversely, T. serpentiformis is considered health-associated. This discrepancy was investigated in a comparative study of the two Tannerella species. The biofilm behavior was analyzed upon their addition and of Porphyromonas gingivalis-each bacterium separately or in combinations-to an in vitro five-species oral model biofilm. Biofilm composition and architecture was analyzed quantitatively using real-time PCR and qualitatively by fluorescence in situ hybridization/confocal laser scanning microscopy, and by scanning electron microscopy. The presence of T. serpentiformis led to a decrease of the total cell number of biofilm bacteria, while P. gingivalis was growth-promoting. This effect was mitigated by T. serpentiformis when added to the biofilm together with P. gingivalis. Notably, T. serpentiformis outcompeted T. forsythia numbers when the two species were simultaneously added to the biofilm compared to biofilms containing T. forsythia alone. Tannerella serpentiformis appeared evenly distributed throughout the multispecies biofilm, while T. forsythia was surface-located. Adhesion and invasion assays revealed that T. serpentiformis was significantly less effective in invading human gingival epithelial cells than T. forsythia. Furthermore, compared to T. forsythia, a higher immunostimulatory potential of human gingival fibroblasts and macrophages was revealed for T. serpentiformis, based on mRNA expression levels of the inflammatory mediators interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 and tumor necrosis factor α, and production of the corresponding proteins. Collectively, these data support the potential of T. serpentiformis to interfere with biological processes relevant to the establishment of periodontitis.
最近发现的细菌坦纳菌属蛇形菌是坦纳菌属福赛斯氏菌的最接近的系统发育相关物，其在口腔生物膜中的存在与牙周炎有关。相反，坦纳菌属蛇形菌被认为与健康有关。在对这两种坦纳菌属的比较研究中，研究了这种差异。分析了在体外五物种口腔模型生物膜中分别添加和同时添加卟啉单胞菌牙龈卟啉单胞菌时，生物膜行为。使用实时 PCR 定量分析生物膜组成和结构，并通过荧光原位杂交/共聚焦激光扫描显微镜和扫描电子显微镜进行定性分析。坦纳菌属蛇形菌的存在导致生物膜细菌总数减少，而牙龈卟啉单胞菌则促进生长。当坦纳菌属蛇形菌与牙龈卟啉单胞菌一起添加到生物膜中时，这种作用会减轻。值得注意的是，当两种细菌同时添加到生物膜中时，与仅含有坦纳菌属福赛斯氏菌的生物膜相比，坦纳菌属蛇形菌的数量超过了坦纳菌属福赛斯氏菌的数量。坦纳菌属蛇形菌均匀分布在多物种生物膜中，而坦纳菌属福赛斯氏菌位于表面。粘附和侵袭实验表明，与坦纳菌属福赛斯氏菌相比，坦纳菌属蛇形菌侵入人牙龈上皮细胞的效果明显较差。此外，与坦纳菌属福赛斯氏菌相比，基于促炎介质白细胞介素 6 (IL-6)、IL-8、单核细胞趋化蛋白 1 和肿瘤坏死因子 α 的 mRNA 表达水平以及相应蛋白的产生，人牙龈成纤维细胞和巨噬细胞对坦纳菌属蛇形菌的免疫刺激潜力更高。总的来说，这些数据支持坦纳菌属蛇形菌干扰与牙周炎建立相关的生物学过程的潜力。
