Department of Biochemistry, Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama IV Road, Patumwan, Bangkok, 10330, Thailand.
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Sci Rep. 2024 Feb 10;14(1):3404. doi: 10.1038/s41598-024-53880-w.
Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB (-) control, TB (+) latent and TB (+) active groups. The differential abundance analysis showed that Haemophilus hemolyticus was decreased, while Prevotella species were increased in the pharyngeal microbiome of TB (+) macaques. In addition, Eubacterium coprostanoligenes in the gut was enriched in TB (+) macaques. Alteration of these bacteria might affect immune regulation and TB severity, but details of mechanisms should be further explored and validated. In summary, microbiota may be associated with host immune regulation and affect TB progression. The findings suggested the potential mechanisms of host-microbes interaction, which may improve the understanding of the role of microbiota and help develop therapeutics for TB in the future.
结核病（TB）是一种由结核分枝杆菌复合体（Mtbc）引起的传染病，它从无症状潜伏性 TB 发展为活动性 TB。微生物组被认为是影响 TB 发病机制的一个潜在因素，但研究有限。本研究使用基于 Oxford Nanopore Technologies 的全长 16S rDNA 扩增子测序，探索了食蟹猴咽-肠微生物组与 TB 分期之间的关系。将总共 71 只猕猴分为 TB（-）对照组、TB（+）潜伏组和 TB（+）活动组。差异丰度分析显示，TB（+）组的咽微生物组中嗜血杆菌属减少，而普雷沃氏菌属增加。此外，TB（+）组的肠道中产粪甾烷真杆菌增加。这些细菌的改变可能影响免疫调节和 TB 的严重程度，但具体机制仍需进一步探索和验证。总之，微生物组可能与宿主免疫调节有关，并影响 TB 的进展。这些发现提示了宿主-微生物相互作用的潜在机制，这可能有助于提高对微生物组作用的理解，并有助于未来开发 TB 的治疗方法。
