Sarkar Subhabrata, Ghosh Arnab, Mohindra Ritin, Thomas Linta, Yadav Devender, Kandpal Harish Chand, Biswal Manisha, Lakshmi P V M, Suri Vikas, Koushal Vipin, Malhotra Pankaj, Ratho R K, Puri G D, Singh Mini P
Department of Virology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, 160012 India.
Department of Internal Medicine, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Virusdisease. 2022 Sep;33(3):236-243. doi: 10.1007/s13337-022-00777-9. Epub 2022 Aug 5.
Environmental surfaces are potential source of SARS-CoV2 transmission. The study assessed the efficacy of hospital disinfection policy and contamination of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2) RNA in COVID management Hospital. Inanimate surfaces from both patient areas (n = 70) and non-patient areas (n = 39) were sampled through surface swabbing and subjected to Reverse transcriptase PCR. Out of the 70 samples collected from the COVID hospital, SARS-CoV2 RNA positivity of 17.5% (7/40) and 6.7% (2/30) was seen in high risk and moderate risk area respectively. Samples from Non COVID related patient area such as CD ward and administrative block were assessed and the SARS CoV-2 RNA positivity was 0% and 10% respectively. Among the total 8 environmental surface samples positive for SARS-CoV2 RNA detected from the area surrounding the SARS-CoV2 infected patients, maximum positivity of 31.8% (7/22) was found among the environmental samples collected around the patients with < 20 Ct value in nasopharyngeal swab samples followed by 3.3% positivity (1/30) around patients with Ct value ranging from 20 to 25 whereas no SARS-CoV2 RNA (0/5) was detected around the patient with > 25 Ct value. Nearly 50% (2/4) of the surface samples came positive from the resident PPE and mobile of the treating doctors which largely elaborates the need for stringent doffing measurement and hand hygiene policy post doffing. The study emphasizes the necessity of frequent and aggressive disinfection policy to prevent nosocomial infection in such high risk areas within close vicinity of the patients.
环境表面是严重急性呼吸综合征冠状病毒2（SARS-CoV-2）传播的潜在来源。本研究评估了新冠管理医院的医院消毒政策的有效性以及SARS-CoV-2 RNA的污染情况。通过表面擦拭对患者区域（n = 70）和非患者区域（n = 39）的无生命表面进行采样，并进行逆转录聚合酶链反应。在从新冠医院收集的70个样本中，高风险区域和中风险区域的SARS-CoV-2 RNA阳性率分别为17.5%（7/40）和6.7%（2/30）。对非新冠相关患者区域（如CD病房和行政楼）的样本进行了评估，SARS-CoV-2 RNA阳性率分别为0%和10%。在从SARS-CoV-2感染患者周围区域检测到的8个SARS-CoV-2 RNA阳性的环境表面样本中，鼻咽拭子样本中Ct值<20的患者周围收集的环境样本中阳性率最高，为31.8%（7/22），其次是Ct值在20至25之间的患者周围阳性率为3.3%（1/30），而Ct值>25的患者周围未检测到SARS-CoV-2 RNA（0/5）。近50%（2/4）的表面样本来自主治医生的驻留个人防护装备和移动设备，这在很大程度上说明了严格的脱卸措施和脱卸后手部卫生政策的必要性。该研究强调了在患者附近的此类高风险区域采取频繁且积极的消毒政策以预防医院感染的必要性。
