miR-579 通过与 CRABP2 结合抑制肺腺癌细胞增殖和转移。
MiR-579 Inhibits Lung Adenocarcinoma Cell Proliferation and Metastasis via Binding to CRABP2.
机构信息
Department of Chemotherapy, The Second Affiliated Hospital of Shandong First Medical University, Tai'an City, Shandong, China.
出版信息
Comput Math Methods Med. 2022 Aug 4;2022:9111681. doi: 10.1155/2022/9111681. eCollection 2022.
BACKGROUND
Lung cancer is the cancer with the highest morbidity and mortality. Lung adenocarcinoma (LUAD) is a subtype of lung cancer. The aim of this study is to explore the functions of miR-579 and CRABP2 in lung adenocarcinoma.
METHODS
Cell counting kit-8 (CCK-8) and colony formation assays were applied to calculate cell proliferative abilities. Transwell assay was utilized to measure cell invasive ability.
RESULTS
MiR-579 is low expressed in LUAD tissues and cell lines. MiR-579 inhibits cell viability and invasion of lung adenocarcinoma. Knockdown of CRABP2 inhibits cell proliferation and invasion of Calu-3 cells. MiR-579 suppresses cell proliferation and invasion by regulating CRABP2 in Calu-3 cells.
CONCLUSION
Our study reveals that miR-579 acts as a tumor suppressor in LUAD and miR-579 can target and regulate the expression of CRABP2 to mediate cell proliferation and invasion. This study indicates that miR-579 has a potential to be a candidate biomarker for the treatment of LUAD.
背景
肺癌是发病率和死亡率最高的癌症。肺腺癌（LUAD）是肺癌的一种亚型。本研究旨在探讨 miR-579 和 CRABP2 在肺腺癌中的作用。
方法
用细胞计数试剂盒-8（CCK-8）和集落形成实验来计算细胞增殖能力。Transwell 实验用于测量细胞侵袭能力。
结果
miR-579 在 LUAD 组织和细胞系中低表达。miR-579 抑制肺腺癌细胞活力和侵袭。CRABP2 的敲低抑制 Calu-3 细胞的增殖和侵袭。miR-579 通过在 Calu-3 细胞中调节 CRABP2 来抑制细胞增殖和侵袭。
结论
本研究表明，miR-579 在 LUAD 中作为一种肿瘤抑制因子发挥作用，miR-579 可以靶向并调节 CRABP2 的表达，从而调节细胞增殖和侵袭。这项研究表明，miR-579 有潜力成为 LUAD 治疗的候选生物标志物。
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