Zhao Jing, Zhou Zhijun, Bai Xuefei, Zhang Dai, Zhang Likui, Wang Jinhui, Wu Beibei, Zhu Jiehua, Yang Zhihui
College of Plant Protection, Hebei Agricultural University, Baoding, China.
Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding, China.
Front Microbiol. 2022 Jul 29;13:943232. doi: 10.3389/fmicb.2022.943232. eCollection 2022.
Potato common scab is a main soil-borne disease of potato that can significantly reduce its quality. At present, it is still a challenge to control potato common scab in the field. To address this problem, the 972 family lactococcin (Lcn972) was screened from HN-Q-8 in this study, and an overexpression system was used to obtain Lcn972, which showed a significant inhibitory effect on , with a minimum inhibitory concentration of 10.58 μg/mL. The stability test showed that Lcn972 is stable against UV radiation and high temperature. In addition, long-term storage at room temperature and 4°C had limited effects on its activity level. The antibacterial activity of Lcn972 was enhanced by Cu and Ca, but decreased by protease K. The protein was completely inactivated by Fe. Cell membrane staining showed that Lcn972 damaged the cell membrane integrity of . Scanning electron microscope (SEM) and transmission electron microscope (TEM) observations revealed that the hyphae of treated with Lcn972 were deformed and adhered, the cell membrane was incomplete, the cytoplasm distribution was uneven, and the cell appeared hollow inside, which led to the death of . In conclusion, we used bacteriocin for controlling potato common scab for the first time in this study, and it provides theoretical support for the further application of bacteriocin in the control of plant diseases.
马铃薯普通疮痂病是马铃薯的一种主要土传病害，会显著降低其品质。目前，在田间控制马铃薯普通疮痂病仍然是一项挑战。为解决这一问题，本研究从HN-Q-8中筛选出972家族乳链菌肽（Lcn972），并利用过表达系统获得Lcn972，其对[具体病原菌未明确写出]表现出显著抑制作用，最低抑菌浓度为10.58μg/mL。稳定性测试表明，Lcn972对紫外线辐射和高温具有稳定性。此外，在室温及4℃下长期储存对其活性水平影响有限。Lcn972的抗菌活性因铜和钙而增强，但被蛋白酶K降低。铁可使该蛋白完全失活。细胞膜染色显示，Lcn972破坏了[具体病原菌未明确写出]的细胞膜完整性。扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察表明，用Lcn972处理后的[具体病原菌未明确写出]菌丝变形并粘连，细胞膜不完整，细胞质分布不均，细胞内部出现空洞，导致[具体病原菌未明确写出]死亡。总之，本研究首次利用细菌素防治马铃薯普通疮痂病，为细菌素在植物病害防治中的进一步应用提供了理论支持。
