Bauss F, Dröge W, Männel D N
Infect Immun. 1987 Jul;55(7):1622-5. doi: 10.1128/iai.55.7.1622-1625.1987.
Endotoxic reactions induced in mice by recombinant human tumor necrosis factor (TNF) were examined. Mice showed a dose-dependent hypothermia after intravenous TNF injection which was similar to a reaction to lipopolysaccharide injection. Plasma glucose levels were decreased, and plasma lactate levels were increased. Blood hematocrit levels were increased after TNF injection. No interleukin-1 activity was detected in the plasma of TNF-treated animals. The number of leukocytes was reduced 30 min after TNF injection and returned to normal within 24 h. Thus, the data demonstrate that the pathophysiological effects induced by TNF were similar to the effects induced by bacterial endotoxin. Since lipopolysaccharide is a very potent agent for eliciting TNF release from activated macrophages, these results suggest that TNF could act as an endogenous mediator of endotoxin effects.
对重组人肿瘤坏死因子（TNF）诱导小鼠产生的内毒素反应进行了研究。静脉注射TNF后，小鼠出现剂量依赖性体温过低，这与注射脂多糖后的反应相似。血浆葡萄糖水平降低，血浆乳酸水平升高。注射TNF后血细胞比容水平升高。在接受TNF治疗的动物血浆中未检测到白细胞介素-1活性。注射TNF后30分钟白细胞数量减少，并在24小时内恢复正常。因此，数据表明TNF诱导的病理生理效应与细菌内毒素诱导的效应相似。由于脂多糖是一种非常有效的从活化巨噬细胞中引发TNF释放的物质，这些结果表明TNF可能作为内毒素效应的内源性介质发挥作用。
