Bartholeyns J, Freudenberg M, Galanos C
Infect Immun. 1987 Sep;55(9):2230-3. doi: 10.1128/iai.55.9.2230-2233.1987.
Lewis lung carcinoma and EMT6 sarcoma growing as solid tumors in mice caused a gradual increase in the susceptibility of the animals to lethal toxicity of endotoxins (lipopolysaccharides [LPS]). By day 15 following inoculation of the tumors, the 50% lethal dose of LPS, which in normal mice was approximately 400 micrograms, decreased to 2 micrograms for the sarcoma-bearing mice and 0.1 microgram for the carcinoma-bearing mice. This sensitization to endotoxin was paralleled by a high sensitization to tumor necrosis factor (TNF). Human recombinant TNF given to normal mice was lethal at about 500 micrograms. It was lethal for 50% of the animals bearing EMT6 or Lewis lung carcinoma tumors in amounts of 4 and 0.01 micrograms, respectively, on day 15 following tumor inoculation. The sensitization of tumor-bearing animals to LPS and TNF was paralleled by marked granulocytosis.
在小鼠体内以实体瘤形式生长的刘易斯肺癌和EMT6肉瘤会使动物对内毒素（脂多糖[LPS]）致死毒性的易感性逐渐增加。在接种肿瘤后的第15天，LPS的半数致死剂量在正常小鼠中约为400微克，而对于携带肉瘤的小鼠降至2微克，对于携带癌的小鼠则降至0.1微克。这种对内毒素的致敏作用与对肿瘤坏死因子（TNF）的高度致敏作用同时出现。给正常小鼠注射人重组TNF，剂量约为500微克时会致死。在接种肿瘤后的第15天，分别给予4微克和0.01微克的人重组TNF，可使50%携带EMT6或刘易斯肺癌肿瘤的动物致死。荷瘤动物对LPS和TNF的致敏作用与明显的粒细胞增多同时出现。
