Dekate Kamlesh, Barpande Suresh, Tupkari Jagdish, Thakur Mansee, Swain Niharika
Department of Oral Pathology, MGM dental College and Hospital, Mumbai, Maharashtra, India.
Department of Education, Ex. Deputy Director of Medical Education and Research, Mumbai, Maharashtra, India.
J Oral Maxillofac Pathol. 2022 Apr-Jun;26(2):284. doi: 10.4103/jomfp.jomfp_70_20. Epub 2022 Jun 28.
Studies established that human cancer is principally a genetic disease; it arises as accumulation of a set of genetic changes. In the pathogenesis of cancer, genetic instability is the sequential event to a carcinogenic stimulus resulting in various genomic changes including DNA damage.
To assess genetic instability, as susceptibility to DNA damage, we used single-cell gel electrophoresis (comet assay) to study double strand breaks in associated with the risk of oral squamous cell carcinoma (OSCC).
We used comet assay to measure double strand break in individual peripheral blood lymphocytes from 50 individuals with OSCC and 30 healthy control subjects. All personal information was gathered from subjects including tobacco history. DNA damage was visualized as comet assay and quantified by movement of damaged strands as length of tail.
Study results of OSCC patients were observed in relation to clinical staging and histological grading of carcinoma. On the basis of clinical observation, cases were grouped in to Stage I, Stage II, Stage III and Stage IV. No stage I cases were in study sample. The mean DNA damage migration length was observed 4.600 ± 0.4613 μm in stage II, whereas in Stage III and Stage IV, it was observed to be 4.961 ± 0.5620 μm and 4.883 ± 0.410 μm, respectively. The DNA damage length in histological grades of squamous cell carcinoma patients in Grade I was 4.6437 ± 0.3061 μm and Grade II was 5.3533 ± 0.3831 μm. In comparison with control group and squamous cell carcinoma group, it was observed in the range of 0.02-0.36 μm and varied from 4.04 to 5.84 μm range, respectively. Thus, the results were statistically significant with the histological grading of OSCC.
Unpaired' test and "ANOVA" test are used for statistics.
Unpaired' test and "ANOVA" test are used for statistics.
The amount of DNA strand breaks in peripheral lymphocytes are measured by comet assay which is associated with relative risk of OSCC.
研究表明，人类癌症主要是一种基因疾病；它是一系列基因变化积累的结果。在癌症发病机制中，基因不稳定是致癌刺激后的连续事件，会导致包括DNA损伤在内的各种基因组变化。
为了评估作为DNA损伤易感性的基因不稳定，我们使用单细胞凝胶电泳（彗星试验）来研究与口腔鳞状细胞癌（OSCC）风险相关的双链断裂。
我们使用彗星试验来测量50例OSCC患者和30名健康对照者外周血单个淋巴细胞中的双链断裂。所有个人信息均从受试者处收集，包括吸烟史。DNA损伤通过彗星试验可视化，并通过受损链的移动作为尾巴长度进行量化。
观察了OSCC患者的研究结果与癌症临床分期和组织学分级的关系。根据临床观察，病例分为I期、II期、III期和IV期。研究样本中没有I期病例。II期平均DNA损伤迁移长度为4.600±0.4613μm，而III期和IV期分别为4.961±0.5620μm和4.883±0.410μm。鳞状细胞癌患者组织学分级中，I级的DNA损伤长度为4.6437±0.3061μm，II级为5.3533±0.3831μm。与对照组和鳞状细胞癌组相比，分别在0.02 - 0.36μm范围内和4.04至5.84μm范围内变化。因此，结果与OSCC的组织学分级具有统计学意义。
使用不成对检验和方差分析进行统计。
通过彗星试验测量外周淋巴细胞中的DNA链断裂量，其与OSCC的相对风险相关。
