Pragallapati Sindhuri, Manyam Ravikanth
Department of Oral and Maxillofacial pathology and Oral Microbiology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.
J Oral Maxillofac Pathol. 2022 Apr-Jun;26(2):185-191. doi: 10.4103/jomfp.jomfp_9_21. Epub 2022 Jun 28.
Facilitative glucose transporters (GLUTs), which mediate glucose transport across the cell membrane, differ in their tissue distribution and affinity for glucose. GLUT1 is ubiquitously present and help in the basal uptake of glucose into the cells. Its expression is known to be elevated in conditions that induce hypoxia and by growth factors. GLUT1 is known to be increased in many malignant tumors to meet the metabolic requirements, but its role in odontogenic tumors is not known.
The objective of this study is to evaluate and compare the immunohistochemical expression of GLUT1 in ameloblastoma (AM) and odontogenic keratocyst (OKC).
Thirty cases each of AM and OKCs were immunohistochemically stained using anti-GLUT1 antibody according to the standard protocol. Qualitative assessment of GLUT1 expression was done under the categories of distribution, intensity and localization of staining. Quantitative assessment was done using Image J software. The results were tabulated and statistically analyzed.
GLUT1 positivity was observed in 25 (83.3%) cases of OKC and 26 (86.7%) of AM cases. The majority of cells in the suprabasal layer of OKC showed positivity, whereas the equal distribution of staining was observed in the central and peripheral cells of AM.
GLUT1 expression in these tumors is suggestive of an increased glucose uptake and probably increased utilization of energy, which may be correlated with their aggressive behavior.
易化型葡萄糖转运蛋白（GLUTs）介导葡萄糖跨细胞膜转运，其在组织分布和对葡萄糖的亲和力方面存在差异。GLUT1广泛存在，有助于细胞对葡萄糖的基础摄取。已知在诱导缺氧的情况下以及生长因子作用下其表达会升高。已知在许多恶性肿瘤中GLUT1会增加以满足代谢需求，但它在牙源性肿瘤中的作用尚不清楚。
本研究的目的是评估和比较GLUT1在成釉细胞瘤（AM）和牙源性角化囊肿（OKC）中的免疫组化表达。
按照标准方案，使用抗GLUT1抗体对30例AM和30例OKC进行免疫组化染色。对GLUT1表达进行定性评估，包括染色的分布、强度和定位类别。使用Image J软件进行定量评估。结果列表并进行统计学分析。
在25例（83.3%）OKC病例和26例（86.7%）AM病例中观察到GLUT1阳性。OKC基底层以上的大多数细胞呈阳性，而在AM的中央和周边细胞中染色分布均匀。
这些肿瘤中GLUT1的表达提示葡萄糖摄取增加，可能能量利用增加，这可能与其侵袭性行为相关。
