Vera-Sirera Beatriz, Forner-Navarro Leopoldo, Vera-Sempere Francisco
Department of Stomatology, University of Valencia, Valencia, Spain.
Department of Stomatology, Endodontics Unit, University of Valencia, Valencia, Spain.
BMC Oral Health. 2016 Mar 10;16:32. doi: 10.1186/s12903-016-0191-2.
Keratin-producing odontogenic cysts (KPOCs) are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. KPOCs included orthokeratinised odontogenic cyst (OOC) and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours (KCOTs). GLUT1 is a protein transporter that is involved in the active uptake of glucose across cell membranes and that is overexpressed in tumours in close correlation with the proliferation rate and positron emission tomography (PET) imaging results.
A series of 58 keratin-producing odontogenic cysts was evaluated histologically and immunohistochemically in terms of GLUT1 expression. Different data were correlated using the beta regression model in relation to histological type and immunohistochemical expression of GLUT1, which was quantified using two different morphological methods.
KPOC cases comprised 12 OOCs and 46 KCOTs, the latter corresponding to 6 syndromic and 40 sporadic KCOTs. GLUT1 expression was very low in OOC cases compared with KCOT cases, with statistical significant differences when quantification was considered. Different GLUT1 localisation patterns were revealed by immunostaining, with the parabasal cells showing higher reactivity in KCOTs. However, among KCOTs cases, GLUT1 expression was unable to establish differences between syndromic and sporadic cases.
GLUT1 expression differentiated between OOC and KCOT cases, with significantly higher expression in KCOTs, but did not differentiate between syndromic and sporadic KCOT cases. However, given the structural characteristics of KCOTs, we hypothesised that PET imaging methodology is probably not a useful diagnostic tool for KCOTs. Further studies of GLUT1 expression and PET examination in KCOT series are needed to confirm this last hypothesis.
产生角蛋白的牙源性囊肿（KPOC）是一组具有侵袭性、高复发率和多灶性的囊性病变。KPOC包括正角化牙源性囊肿（OOC）和不全角化牙源性囊肿，现被认为是真正的肿瘤，称为角化囊性牙源性肿瘤（KCOT）。葡萄糖转运蛋白1（GLUT1）是一种蛋白质转运体，参与葡萄糖跨细胞膜的主动摄取，在肿瘤中过度表达，与增殖率和正电子发射断层扫描（PET）成像结果密切相关。
对一系列58例产生角蛋白的牙源性囊肿进行组织学和免疫组织化学评估，观察GLUT1表达情况。使用β回归模型将不同数据与组织学类型和GLUT1的免疫组织化学表达相关联，GLUT1表达通过两种不同的形态学方法进行量化。
KPOC病例包括12例OOC和46例KCOT，后者包括6例综合征性KCOT和40例散发性KCOT。与KCOT病例相比，OOC病例中GLUT1表达非常低，在考虑量化时具有统计学显著差异。免疫染色显示不同的GLUT1定位模式，基底旁细胞在KCOT中显示出更高的反应性。然而，在KCOT病例中，GLUT1表达无法区分综合征性和散发性病例。
GLUT1表达可区分OOC和KCOT病例，KCOT中的表达明显更高，但不能区分综合征性和散发性KCOT病例。然而，鉴于KCOT的结构特征，我们推测PET成像方法可能不是KCOT的有用诊断工具。需要对KCOT系列中的GLUT1表达和PET检查进行进一步研究以证实这一假设。
