Kontro P, Oja S S
Int J Neurosci. 1987 Feb;32(3-4):881-9. doi: 10.3109/00207458709043343.
The sodium-independent binding of taurine and GABA were determined with mouse brain membranes subjected to various freezing-thawing cycles, washing procedures and Triton X-100 treatments. The treated membranes were characterized by electron microscopy and determination of their content of endogenous free amino acids, e.g., taurine, GABA, glutamate, aspartate and glycine. The washings and the detergent gradually disrupted all synaptic structures, yielding empty-looking sealed membrane pouches of various sizes. Only traces of taurine, GABA, aspartate and glutamate were present in the Triton X-100-treated membranes, whereas these membranes still contained some glycine. Concomitantly, the affinity and maximal capacity of GABA binding increased, indicating that the treatments progressively removed some endogenous GABA binding inhibitors. Saturable taurine binding became detectable only after the Triton X-100 treatment, endogenous taurine, removable from membranes only with difficulty, obviously hampering binding measurements.
采用经过不同冻融循环、洗涤程序和Triton X-100处理的小鼠脑膜，测定牛磺酸和γ-氨基丁酸（GABA）的非钠依赖性结合。通过电子显微镜和测定其内源性游离氨基酸（如牛磺酸、GABA、谷氨酸、天冬氨酸和甘氨酸）的含量来表征处理后的脑膜。洗涤和去污剂逐渐破坏所有突触结构，产生各种大小的看似空的密封膜袋。在经Triton X-100处理的脑膜中仅存在痕量的牛磺酸、GABA、天冬氨酸和谷氨酸，而这些脑膜仍含有一些甘氨酸。同时，GABA结合的亲和力和最大容量增加，表明这些处理逐渐去除了一些内源性GABA结合抑制剂。仅在Triton X-100处理后才检测到可饱和的牛磺酸结合，内源性牛磺酸仅难以从膜上去除，明显妨碍结合测量。
