Kontro P, Oja S S
Adv Biochem Psychopharmacol. 1983;37:23-34.
An account is given of our endeavours to demonstrate sodium-independent and -dependent taurine binding to brain synaptic membranes. The total and specific sodium-dependent binding and the total sodium-independent binding were fairly easily amenable to characterization, but the disclosure of specific sodium-independent binding succeeded only after repeated freezing and thawing, thorough buffer washes and two Triton X-100 treatments of the membranes. The sodium-independent binding capacity for taurine was rather small and only one type of relatively low-affinity binding site was revealed.
本文介绍了我们为证明牛磺酸与脑突触膜的钠非依赖性和钠依赖性结合所做的努力。总钠依赖性结合、特异性钠依赖性结合以及总钠非依赖性结合都比较容易进行表征，但特异性钠非依赖性结合的揭示仅在对膜进行反复冻融、充分的缓冲液洗涤以及两次Triton X-100处理之后才成功。牛磺酸的钠非依赖性结合能力相当小，并且仅揭示了一种亲和力相对较低的结合位点。
