Kontro P, Oja S S
Prog Clin Biol Res. 1985;179:249-59.
The sodium-independent taurine binding to twice Triton treated brain membranes characterized here was reversible, temperature-insensitive and saturable with a broad pH optimum, being thus characteristic of a synaptic receptor site. Only one type of binding site with a rather low maximal capacity was detected. Furthermore, binding exhibited properties of positive cooperativity, indicating that two or more taurine molecules possibly interact at a binding site. Both GABA and glycine as well as their agonists and antagonists strongly displaced taurine binding. The novel anticonvulsive taurine derivatives wee also effective displacers. The relevance of the binding observed in vitro to the possible synaptic receptors for taurine in vivo still remains to be determined.
此处所描述的与经两次Triton处理的脑膜结合的不依赖钠的牛磺酸结合是可逆的、对温度不敏感且可饱和的，具有较宽的pH最佳值，因此是突触受体位点的特征。仅检测到一种具有相当低最大容量的结合位点。此外，结合表现出正协同性，表明两个或更多牛磺酸分子可能在一个结合位点相互作用。GABA和甘氨酸及其激动剂和拮抗剂都强烈取代了牛磺酸结合。新型抗惊厥牛磺酸衍生物也是有效的取代剂。体外观察到的结合与体内牛磺酸可能的突触受体的相关性仍有待确定。
